EP 1080200 A1 20010307 - DIFFERENTIAL CYTOTOXICITY OF ALTERNATIVE FORMS OF ROTAVIRUS NONSTRUCTURAL PROTEIN 4
Title (en)
DIFFERENTIAL CYTOTOXICITY OF ALTERNATIVE FORMS OF ROTAVIRUS NONSTRUCTURAL PROTEIN 4
Title (de)
UNTERSCHIEDLICHE ZYTOTOXIZITÄT ALTERNATIVER FORMEN DES ROTAVIRALEN NICHTSTRUKTURPROTEINS 4
Title (fr)
CYTOTOXICITE DIFFERENTIELLE DE FORMES ALTERNATIVES DE LA PROTEINE 4 NON STRUCTURALE DU ROTAVIRUS
Publication
Application
Priority
- US 9911872 W 19990528
- US 8732098 P 19980529
Abstract (en)
[origin: WO9961621A1] The nonstructural protein 4 (NSP4) in the SA11 ATCC rotavirus strain has a histidine at amino acid position 47. This substituted form is more cytotoxic than the NSP4 of the Australia rotavirus strain, which has an asparagine at amino acid position 47. The histidine at amino acid position 47 is mutagenized to another amino acid to produce an alternative form of NSP4 which has reduced toxicity, while retaining its antigenicity and immunogenicity. NSP4 having a glutamic acid at amino acid position 48 is more cytotoxic than NSP4 having a lysine at amino acid position 48. The lysine at amino acid position 48 is mutagenized to another amino acid other than glutamic acid to produce an alternative form of NSP4 which has reduced toxicity, while retaining its antigenicity and immunogenicity.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); A61K 35/76 (2006.01); A61K 38/00 (2006.01); A61K 39/15 (2006.01); A61K 39/385 (2006.01); A61P 1/12 (2006.01); A61P 43/00 (2006.01); C07K 14/14 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 7/04 (2006.01); C12N 15/46 (2006.01); C12P 21/02 (2006.01); A61K 39/00 (2006.01)
CPC (source: EP KR US)
A61P 1/12 (2018.01 - EP); A61P 43/00 (2018.01 - EP); C07K 14/005 (2013.01 - EP); C12N 15/11 (2013.01 - KR); A61K 39/00 (2013.01 - EP KR US); C12N 2710/14143 (2013.01 - EP); C12N 2720/12322 (2013.01 - EP)
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU NL PT SE
DOCDB simple family (publication)
WO 9961621 A1 19991202; AU 4216499 A 19991213; AU 771127 B2 20040311; BR 9910763 A 20010213; CA 2328451 A1 19991202; CN 1208463 C 20050629; CN 1302330 A 20010704; EP 1080200 A1 20010307; IL 139997 A0 20020210; JP 2002516106 A 20020604; KR 20010043868 A 20010525
DOCDB simple family (application)
US 9911872 W 19990528; AU 4216499 A 19990528; BR 9910763 A 19990528; CA 2328451 A 19990528; CN 99806546 A 19990528; EP 99925989 A 19990528; IL 13999799 A 19990528; JP 2000551005 A 19990528; KR 20007013353 A 20001127