Global patent index - EP 1080210 A2

EP 1080210 A2 2001-03-07 - METHODS FOR PRODUCING A POLYPEPTIDE BY MODIFYING THE COPY NUMBER OF A GENE

Title (en)

METHODS FOR PRODUCING A POLYPEPTIDE BY MODIFYING THE COPY NUMBER OF A GENE

Title (de)

METHODEN ZUR HERSTELLUNG EINES POLYPEPTIDES DURCH MODIFIZIERUNG DER KOPIEZAHL DES GENS

Title (fr)

PROCEDE DE PRODUCTION D'UN POLYPEPTIDE EN MODIFIANT LE NOMBRE DE COPIES D'UN GENE

Publication

EP 1080210 A2 (EN)

Application

EP 99925956 A

Priority

  • US 9911816 W
  • US 8550598 A

Abstract (en)

[origin: WO9961651A2] The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant cell under conditions conducive for production of the polypeptide, wherein (i) the mutant cell is related to a parent cell, which parent cell comprises at least two tandem copies of a nucleic acid sequence encoding the polypeptide or a nucleic acid sequence encoding the polypeptide, which nucleic acid sequence comprises repeat sequences at the 5' and 3' ends of the nucleic acid sequence, by the introduction of a nucleic acid construct into the genome of the parent cell at a locus which is within or not within the nucleic acid sequence(s), wherein the introduction of the nucleic acid construct into the locus modifies the copy number of the nucleic acid sequence(s) and the modification of the copy number is not a result of selective pressure; and (ii) the mutant cell produces more or less of the polypeptide than the parent cell when both cells are cultivated under the same conditions; and (b) recovering the poylpeptide from the cultivation medium. The present invention also relates to methods for obtaining a mutant cell and mutant cells.

IPC 1-7 (main, further and additional classification)

C12N 15/67; C12N 15/69; C12N 15/55; C12N 15/56; C12N 15/90; C12N 9/20; C12N 9/42; C12N 1/14; C12P 21/02

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 9/20 (2006.01); C12N 9/42 (2006.01); C12N 15/69 (2006.01); C12N 15/90 (2006.01); C12P 21/02 (2006.01)

CPC (invention and additional information)

C12N 9/2437 (2013.01); C12N 9/20 (2013.01); C12N 15/69 (2013.01); C12N 15/902 (2013.01); C12P 21/02 (2013.01); C12Y 302/01004 (2013.01)

Designated contracting state (EPC)

AT BE CH DE DK ES FI FR GB GR IE IT LI NL PT SE

EPO simple patent family

WO 9961651 A2 19991202; WO 9961651 A3 20000302; AU 4213999 A 19991213; CN 1307642 A 20010808; EP 1080210 A2 20010307; JP 2002516116 A 20020604

INPADOC legal status

2005-07-13 [18D] DEEMED TO BE WITHDRAWN

- Ref Legal Event Code: 18D

- Effective date: 20041201

2003-11-05 [17Q] FIRST EXAMINATION REPORT

- Ref Legal Event Code: 17Q

- Effective date: 20030919

2001-11-21 [RAP1] TRANSFER OF RIGHTS OF AN EP PUBLISHED APPLICATION

- Owner name: NOVOZYMES BIOTECH, INC.

- Ref Legal Event Code: RAP1

2001-03-07 [17P] REQUEST FOR EXAMINATION FILED

- Ref Legal Event Code: 17P

- Effective date: 20001113

2001-03-07 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): AT BE CH DE DK ES FI FR GB GR IE IT LI NL PT SE