Global patent index - EP 1082461 A1

EP 1082461 A1 2001-03-14 - METHOD FOR AMPLIFYING AT LEAST A PARTICULAR NUCLEOTIDE SEQUENCE AND PRIMERS USED

Title (en)

METHOD FOR AMPLIFYING AT LEAST A PARTICULAR NUCLEOTIDE SEQUENCE AND PRIMERS USED

Title (de)

AMPLIFIZIERUNGSVERFAHREN FÜR MINDESTENS EINE SPEZIFISCHE NUKLEOTIDSEQUENZ UND GEEIGNETE PRIMER ZUR AUSFÜHRUNG

Title (fr)

PROCEDE D'AMPLIFICATION D'AU MOINS UNE SEQUENCE NUCLEOTIDIQUE PARTICULIERE ET AMORCES DE MISE EN OEUVRE

Publication

EP 1082461 A1 (FR)

Application

EP 99922230 A

Priority

  • FR 9901247 W
  • FR 9806866 A

Abstract (en)

[origin: FR2779154A1] The invention concerns a method for amplifying at least a particular nucleotide sequence of a synthetic or natural nucleic acid contained in a reaction mixture, the reaction mixture consisting of at least a nucleic acid comprising at least two related nucleotide sequences and/or at least two nucleic acids each comprising at least a related nucleotide sequence; the method uses amplifying primers capable of hybridising with the nucleic acid for amplifying related nucleotide sequences. The invention also concerns primers for implementing such a method. Said method consists in adding, in the reaction mixture, at least a sequence acting as blocking primer capable of: hybridising with at least a nucleotide sequence, which is not the particular nucleotide sequence or sequences to be amplified; preventing at its level the elongation of the amplifying primer. The invention is particularly applicable in the field of techniques for amplifying related genes.

IPC 1-7 (main, further and additional classification)

C12Q 1/68

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); C12Q 1/68 (2006.01)

CPC (invention and additional information)

C12Q 1/6848 (2013.01); C12Q 1/6853 (2013.01)

Combination set (CPC)

  1. C12Q 1/6848 + C12Q 2537/162
  2. C12Q 1/6853 + C12Q 2537/163

Citation (search report)

See references of WO 9961661A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

FR 2779154 A1 19991203; FR 2779154 B1 20020712; AU 3935599 A 19991213; EP 1082461 A1 20010314; JP 2002516666 A 20020611; WO 9961661 A1 19991202

INPADOC legal status

2004-10-06 [18W] WITHDRAWN

- Ref Legal Event Code: 18W

- Effective date: 20040809

2001-03-14 [17P] REQUEST FOR EXAMINATION FILED

- Ref Legal Event Code: 17P

- Effective date: 20001128

2001-03-14 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE