EP 1082461 A1 20010314 - METHOD FOR AMPLIFYING AT LEAST A PARTICULAR NUCLEOTIDE SEQUENCE AND PRIMERS USED
Title (en)
METHOD FOR AMPLIFYING AT LEAST A PARTICULAR NUCLEOTIDE SEQUENCE AND PRIMERS USED
Title (de)
AMPLIFIZIERUNGSVERFAHREN FÜR MINDESTENS EINE SPEZIFISCHE NUKLEOTIDSEQUENZ UND GEEIGNETE PRIMER ZUR AUSFÜHRUNG
Title (fr)
PROCEDE D'AMPLIFICATION D'AU MOINS UNE SEQUENCE NUCLEOTIDIQUE PARTICULIERE ET AMORCES DE MISE EN OEUVRE
Publication
Application
Priority
- FR 9901247 W 19990527
- FR 9806866 A 19980527
Abstract (en)
[origin: FR2779154A1] The invention concerns a method for amplifying at least a particular nucleotide sequence of a synthetic or natural nucleic acid contained in a reaction mixture, the reaction mixture consisting of at least a nucleic acid comprising at least two related nucleotide sequences and/or at least two nucleic acids each comprising at least a related nucleotide sequence; the method uses amplifying primers capable of hybridising with the nucleic acid for amplifying related nucleotide sequences. The invention also concerns primers for implementing such a method. Said method consists in adding, in the reaction mixture, at least a sequence acting as blocking primer capable of: hybridising with at least a nucleotide sequence, which is not the particular nucleotide sequence or sequences to be amplified; preventing at its level the elongation of the amplifying primer. The invention is particularly applicable in the field of techniques for amplifying related genes.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6848 (2018.01); C12Q 1/6853 (2018.01)
CPC (source: EP)
C12Q 1/6848 (2013.01); C12Q 1/6853 (2013.01)
Citation (search report)
See references of WO 9961661A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
FR 2779154 A1 19991203; FR 2779154 B1 20020712; AU 3935599 A 19991213; EP 1082461 A1 20010314; JP 2002516666 A 20020611; WO 9961661 A1 19991202
DOCDB simple family (application)
FR 9806866 A 19980527; AU 3935599 A 19990527; EP 99922230 A 19990527; FR 9901247 W 19990527; JP 2000551041 A 19990527