EP 1082602 A1 20010314 - LUMINESCENCE ASSAY USING CYCLICAL EXCITATION WAVELENGTH SEQUENCE
Title (en)
LUMINESCENCE ASSAY USING CYCLICAL EXCITATION WAVELENGTH SEQUENCE
Title (de)
LUMINESZENZTEST MIT VERWENDUNG EINER ZYKLISCHEN FOLGE VON ANREGUNGSWELLENLÄNGEN
Title (fr)
DOSAGE DE LUMINESCENCE UTILISANT UNE SEQUENCE DE LONGUEUR D'ONDE D'EXCITATION CYCLIQUE
Publication
Application
Priority
- GB 9901512 W 19990601
- GB 9811483 A 19980529
Abstract (en)
[origin: WO9963327A1] A method for the conduct of a measurement of proximity between luminescent species based on detection of transfer of excitation energy between them. A first photoluminescent species (the "donor") and a second photoluminescent species (the "acceptor") are provided and are such that the donor species and the acceptor species have at least some excitation spectral regions which differ and that at least a part of the emission spectrum of the donor overlaps with at least a part of the excitation spectrum of the acceptor. The donor species is excited with a cyclical temporal sequence of wavelength bands, optionally provided as pulses or modulated in intensity, giving rise to a characteristic temporal fluctuation in emission therefrom and emission in at least one wavelength band characteristic of the acceptor is analysed to detect the presence of the said characteristic fluctuation or a subcomponent thereof and optionally also to detect a fluctuation characteristic of direct excitation of the acceptor.
IPC 1-7
IPC 8 full level
G01N 21/64 (2006.01)
CPC (source: EP US)
G01N 21/6408 (2013.01 - EP US); G01N 21/6428 (2013.01 - EP US); G01N 2021/6441 (2013.01 - EP US)
Citation (search report)
See references of WO 9963327A1
Designated contracting state (EPC)
CH DE FR GB LI NL
DOCDB simple family (publication)
WO 9963327 A1 19991209; AU 4153099 A 19991220; EP 1082602 A1 20010314; GB 9811483 D0 19980729; US 6444476 B1 20020903
DOCDB simple family (application)
GB 9901512 W 19990601; AU 4153099 A 19990601; EP 99925138 A 19990601; GB 9811483 A 19980529; US 70147101 A 20010226