Global Patent Index - EP 1082602 A1

EP 1082602 A1 2001-03-14 - LUMINESCENCE ASSAY USING CYCLICAL EXCITATION WAVELENGTH SEQUENCE

Title (en)

LUMINESCENCE ASSAY USING CYCLICAL EXCITATION WAVELENGTH SEQUENCE

Title (de)

LUMINESZENZTEST MIT VERWENDUNG EINER ZYKLISCHEN FOLGE VON ANREGUNGSWELLENLÄNGEN

Title (fr)

DOSAGE DE LUMINESCENCE UTILISANT UNE SEQUENCE DE LONGUEUR D'ONDE D'EXCITATION CYCLIQUE

Publication

EP 1082602 A1 (EN)

Application

EP 99925138 A

Priority

  • GB 9901512 W
  • GB 9811483 A

Abstract (en)

[origin: WO9963327A1] A method for the conduct of a measurement of proximity between luminescent species based on detection of transfer of excitation energy between them. A first photoluminescent species (the "donor") and a second photoluminescent species (the "acceptor") are provided and are such that the donor species and the acceptor species have at least some excitation spectral regions which differ and that at least a part of the emission spectrum of the donor overlaps with at least a part of the excitation spectrum of the acceptor. The donor species is excited with a cyclical temporal sequence of wavelength bands, optionally provided as pulses or modulated in intensity, giving rise to a characteristic temporal fluctuation in emission therefrom and emission in at least one wavelength band characteristic of the acceptor is analysed to detect the presence of the said characteristic fluctuation or a subcomponent thereof and optionally also to detect a fluctuation characteristic of direct excitation of the acceptor.

IPC 1-7 (main, further and additional classification)

G01N 21/64

IPC 8 full level (invention and additional information)

G01N 21/64 (2006.01)

CPC (invention and additional information)

G01N 21/6408 (2013.01); G01N 21/6428 (2013.01); G01N 2021/6441 (2013.01)

Citation (search report)

See references of WO 9963327A1

Designated contracting state (EPC)

CH DE FR GB LI NL

EPO simple patent family

WO 9963327 A1 19991209; AU 4153099 A 19991220; EP 1082602 A1 20010314; GB 9811483 D0 19980729; US 6444476 B1 20020903

INPADOC legal status


2004-07-14 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20040102

2003-01-02 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20021119

2001-03-14 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20001222

2001-03-14 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): CH DE FR GB LI NL