EP 1084276 A1 20010321 - SUPPORT FOR THE PARALLEL IDENTIFICATION AND ESTABLISHMENT OF TRANSCRIPTION PROFILES OF POLYNUCLEIC ACIDS
Title (en)
SUPPORT FOR THE PARALLEL IDENTIFICATION AND ESTABLISHMENT OF TRANSCRIPTION PROFILES OF POLYNUCLEIC ACIDS
Title (de)
TRÄGER FÜR DIE PARALLELE IDENTIFIZIERUNG UND ERSTELLUNG VON TRANSKRIPTIONSPROFILEN VON POLYNUCLEINSÄUREN
Title (fr)
SUPPORT POUR L'IDENTIFICATION ET L'ETABLISSEMENT EN PARALLELE DE PROFILS DE TRANSCRIPTION D'ACIDES POLYNUCLEIQUES
Publication
Application
Priority
- EP 99931052 A 19990610
- DE 19825899 A 19980610
- EP 9904014 W 19990610
- EP 98110608 A 19980610
Abstract (en)
[origin: WO9964623A1] The invention relates to a support. Oligonucleotides or polynucleotides are covalently bound with the 5'- or 3'-termination on least one main surface of said support via bifunctional spacers and bifunctional linkers. The support is characterized in that the oligonucleotides or polynucleotides which are covalently bound with the 5'- or 3'-termination via bifunctional spacers and bifunctional linkers comprise 200 to 600 bp, and the oligonucleotides or polynucleotides can be obtained by using a method which comprises the following steps: Selecting homologous regions of mRNA of a target species and of at least one model species; selecting amplification primers which permit the amplification of 200 to 600, preferably 200 to 400 bp long nucleic acids from the homologous regions of both the mRNA of the target species and the mRNA of at least one model species, whereby the amplification primers optionally comprise a maximum of 1 mismatch per 6 nucleic acids of the amplification primer; immobilizing the nucleic acids on at the least one main surface of the support, said nucleic acids being obtained from the corresponding 200 to 600 bp long nucleic acids which are amplified for the target species or for the at least one model species by amplifications using the amplification primers.
IPC 1-7
IPC 8 full level
C07B 61/00 (2006.01); C07H 21/00 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C40B 40/06 (2006.01)
CPC (source: EP US)
C07H 21/00 (2013.01 - EP US); C12Q 1/6834 (2013.01 - EP US); B01J 2219/00529 (2013.01 - EP US); B01J 2219/00608 (2013.01 - EP US); B01J 2219/00612 (2013.01 - EP US); B01J 2219/00626 (2013.01 - EP US); B01J 2219/00637 (2013.01 - EP US); B01J 2219/00722 (2013.01 - EP US); C07B 2200/11 (2013.01 - EP US); C40B 40/06 (2013.01 - EP US)
Citation (search report)
See references of WO 9964623A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9964623 A1 19991216; AU 4771199 A 19991230; CA 2330478 A1 19991216; EP 1084276 A1 20010321; JP 2002517257 A 20020618; US 6830886 B1 20041214
DOCDB simple family (application)
EP 9904014 W 19990610; AU 4771199 A 19990610; CA 2330478 A 19990610; EP 99931052 A 19990610; JP 2000553613 A 19990610; US 70158401 A 20010201