EP 1086239 A1 20010328 - FORMULATIONS FOR ELECTROPORATION
Title (en)
FORMULATIONS FOR ELECTROPORATION
Title (de)
FORMULIERUNGEN FÜR DIE ELEKTROPORATION
Title (fr)
FORMULATIONS POUR ELECTROPORATION
Publication
Application
Priority
- US 9911927 W 19990528
- US 8869198 P 19980608
Abstract (en)
[origin: WO9964615A1] A novel method is provided for delivering nucleic acid molecules to the cells of an organism by pulse voltage delivery. The method involves the combination of formulated nucleic acid molecules with devices for injecting the molecules by pulse voltage or an electrical field. Disclosed are compositions and methods for enhancing the administration to and uptake of nucleic acids in a mammal. The methods disclosed provide an increased transfection and/or gene delivery efficiency by enhancing the uptake of formulated nucleic acid molecules by applying an electrical field which destabilizes the cellular membrane thereby opening pores or passageways which allow extracellular material to be introduced to the cell. Also disclosed are examples which demonstate that the combination of formulated nucleic acid molecules and pulse voltage injection methods results in immune responses which are superior to those obtained by conventional means of delivery. Methods for delivery, as well as methods for formulating nucleic acid molecules with various compounds, such as cationic complexing agents, polymeric and non-polymeric formulations, protective, interactive, non-condensing systems are also disclosed.
IPC 1-7
IPC 8 full level
A61K 9/00 (2006.01); A61K 9/127 (2006.01); A61K 31/7105 (2006.01); A61K 31/711 (2006.01); A61K 38/27 (2006.01); A61K 39/00 (2006.01); A61K 39/29 (2006.01); A61K 47/32 (2006.01); A61K 47/42 (2006.01); A61K 47/44 (2006.01); A61K 48/00 (2006.01); A61P 1/16 (2006.01); A61P 5/00 (2006.01); A61P 35/00 (2006.01); C12M 1/00 (2006.01); C12N 13/00 (2006.01); C12N 15/09 (2006.01); C12N 15/87 (2006.01)
CPC (source: EP US)
A61K 48/00 (2013.01 - EP US); A61P 1/16 (2017.12 - EP); A61P 5/00 (2017.12 - EP); A61P 35/00 (2017.12 - EP); C12N 13/00 (2013.01 - EP US); C12N 15/87 (2013.01 - EP US)
Citation (search report)
See references of WO 9964615A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9964615 A1 19991216; AU 4219399 A 19991230; CA 2330211 A1 19991216; EP 1086239 A1 20010328; JP 2002517519 A 20020618; US 2002025578 A1 20020228; US 2002102729 A1 20020801
DOCDB simple family (application)
US 9911927 W 19990528; AU 4219399 A 19990528; CA 2330211 A 19990528; EP 99926020 A 19990528; JP 2000553605 A 19990528; US 32260299 A 19990528; US 90300901 A 20010710