Global patent index - EP 1086239 A1

EP 1086239 A1 2001-03-28 - FORMULATIONS FOR ELECTROPORATION

Title (en)

FORMULATIONS FOR ELECTROPORATION

Title (de)

FORMULIERUNGEN FÜR DIE ELEKTROPORATION

Title (fr)

FORMULATIONS POUR ELECTROPORATION

Publication

EP 1086239 A1 (EN)

Application

EP 99926020 A

Priority

  • US 9911927 W
  • US 8869198 P

Abstract (en)

[origin: WO9964615A1] A novel method is provided for delivering nucleic acid molecules to the cells of an organism by pulse voltage delivery. The method involves the combination of formulated nucleic acid molecules with devices for injecting the molecules by pulse voltage or an electrical field. Disclosed are compositions and methods for enhancing the administration to and uptake of nucleic acids in a mammal. The methods disclosed provide an increased transfection and/or gene delivery efficiency by enhancing the uptake of formulated nucleic acid molecules by applying an electrical field which destabilizes the cellular membrane thereby opening pores or passageways which allow extracellular material to be introduced to the cell. Also disclosed are examples which demonstate that the combination of formulated nucleic acid molecules and pulse voltage injection methods results in immune responses which are superior to those obtained by conventional means of delivery. Methods for delivery, as well as methods for formulating nucleic acid molecules with various compounds, such as cationic complexing agents, polymeric and non-polymeric formulations, protective, interactive, non-condensing systems are also disclosed.

IPC 1-7 (main, further and additional classification)

C12N 15/87; A61K 48/00

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); A61K 9/00 (2006.01); A61K 9/127 (2006.01); A61K 31/7105 (2006.01); A61K 31/711 (2006.01); A61K 38/27 (2006.01); A61K 39/00 (2006.01); A61K 39/29 (2006.01); A61K 47/32 (2006.01); A61K 47/42 (2006.01); A61K 47/44 (2006.01); A61K 48/00 (2006.01); A61P 1/16 (2006.01); A61P 5/00 (2006.01); A61P 35/00 (2006.01); C12M 1/00 (2006.01); C12N 13/00 (2006.01); C12N 15/87 (2006.01)

CPC (invention and additional information)

C12N 15/87 (2013.01); A61K 48/00 (2013.01); C12N 13/00 (2013.01)

Citation (search report)

See references of WO 9964615A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9964615 A1 19991216; AU 4219399 A 19991230; CA 2330211 A1 19991216; EP 1086239 A1 20010328; JP 2002517519 A 20020618; US 2002025578 A1 20020228; US 2002102729 A1 20020801

INPADOC legal status

2004-06-09 [18D] DEEMED TO BE WITHDRAWN

- Ref Legal Event Code: 18D

- Effective date: 20031202

2001-03-28 [17P] REQUEST FOR EXAMINATION FILED

- Ref Legal Event Code: 17P

- Effective date: 20001222

2001-03-28 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE