Global Patent Index - EP 1086242 A1

EP 1086242 A1 20010328 - FLUORESCENT ASSAY FOR TOPOISOMERASE INHIBITORS

Title (en)

FLUORESCENT ASSAY FOR TOPOISOMERASE INHIBITORS

Title (de)

FLUORESZENEBESTIMMUNG AUF TOPOISOMERASE INHIBITOREN

Title (fr)

METHODE DE DETECTION PAR FLUORESCENCE D'INHIBITEURS DE TOPOISOMERASE

Publication

EP 1086242 A1 20010328 (EN)

Application

EP 99942591 A 19990322

Priority

  • IB 9900473 W 19990322
  • US 7926598 P 19980325

Abstract (en)

[origin: WO9949077A1] The subject invention involves methods for determining the activity of test compounds as topoisomerase inhibitors comprising mixing, in a fluid, a test compound, a DNA, and a topoisomerase; incubating the fluid; then incorporating a cyanine nucleic acid dye such as PicoGreen3, measuring the fluorescence, and comparing to the fluorescence from controls.

IPC 1-7

C12Q 1/533; G01N 33/58

IPC 8 full level

G01N 33/536 (2006.01); C12Q 1/533 (2006.01); C12Q 1/68 (2006.01); G01N 33/573 (2006.01); G01N 33/58 (2006.01)

CPC (source: EP)

C12Q 1/533 (2013.01); G01N 33/582 (2013.01); G01N 2333/99 (2013.01)

Citation (search report)

See references of WO 9949077A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU NL PT SE

DOCDB simple family (publication)

WO 9949077 A1 19990930; AU 3269999 A 19991018; CA 2324344 A1 19990930; EP 1086242 A1 20010328; IL 138532 D0 20011031; JP 2002507433 A 20020312; NO 20004753 D0 20000922; NO 20004753 L 20001123

DOCDB simple family (application)

IB 9900473 W 19990322; AU 3269999 A 19990322; CA 2324344 A 19990322; EP 99942591 A 19990322; IL 13853299 A 19990322; JP 2000538035 A 19990322; NO 20004753 A 20000922