Global Patent Index - EP 1086242 A1

EP 1086242 A1 2001-03-28 - FLUORESCENT ASSAY FOR TOPOISOMERASE INHIBITORS

Title (en)

FLUORESCENT ASSAY FOR TOPOISOMERASE INHIBITORS

Title (de)

FLUORESZENEBESTIMMUNG AUF TOPOISOMERASE INHIBITOREN

Title (fr)

METHODE DE DETECTION PAR FLUORESCENCE D'INHIBITEURS DE TOPOISOMERASE

Publication

EP 1086242 A1 (EN)

Application

EP 99942591 A

Priority

  • IB 9900473 W
  • US 7926598 P

Abstract (en)

[origin: WO9949077A1] The subject invention involves methods for determining the activity of test compounds as topoisomerase inhibitors comprising mixing, in a fluid, a test compound, a DNA, and a topoisomerase; incubating the fluid; then incorporating a cyanine nucleic acid dye such as PicoGreen3, measuring the fluorescence, and comparing to the fluorescence from controls.

IPC 1-7 (main, further and additional classification)

C12Q 1/533; G01N 33/58

IPC 8 full level (invention and additional information)

G01N 33/536 (2006.01); C12Q 1/533 (2006.01); C12Q 1/68 (2006.01); G01N 33/573 (2006.01); G01N 33/58 (2006.01)

CPC (invention and additional information)

C12Q 1/533 (2013.01); G01N 33/582 (2013.01); G01N 2333/99 (2013.01)

Citation (search report)

See references of WO 9949077A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU NL PT SE

EPO simple patent family

WO 9949077 A1 19990930; AU 3269999 A 19991018; CA 2324344 A1 19990930; EP 1086242 A1 20010328; IL 138532 D0 20011031; JP 2002507433 A 20020312; NO 20004753 A 20001123; NO 20004753 D0 20000922

INPADOC legal status


2003-04-09 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20020930

2001-03-28 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20001227

2001-03-28 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU NL PT SE