EP 1086244 A1 20010328 - RESTRICTION ENZYME GENE DISCOVERY METHOD
Title (en)
RESTRICTION ENZYME GENE DISCOVERY METHOD
Title (de)
METHODE ZUR ERKENNUNG DER GENE VON RESTRIKTIONSENZYMEN
Title (fr)
DECOUVERTE DE GENES D'ENZYMES DE RESTRICTION
Publication
Application
Priority
- US 9913295 W 19990611
- US 8908698 P 19980612
- US 8910198 P 19980612
Abstract (en)
[origin: WO9964632A1] The invention is directed to direct cloning of intact genes, with a high probability that the orientation of expression is known in advance, and with a low probability of being associated with extraneous possibly toxic genes. The invention is particularly directed to obtaining genes encoded in DNA cassettes comprised of repeat sequences flanking variable open reading frames. The invention encompasses obtaining such cassette-encoded genes using oligonucleotides hybridizing to the repeated elements, cloning them and expressing them. Expression may employ tightly regulated vectors and useful strains disclosed. Methods for identifying restriction endonuclease and DNA methyltransferase genes in the absence of prior information about the sequences or biochemical specificities of these are also disclosed.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP)
C12N 15/10 (2013.01); C12N 15/1034 (2013.01)
Designated contracting state (EPC)
DE FR GB
DOCDB simple family (publication)
WO 9964632 A1 19991216; EP 1086244 A1 20010328; EP 1086244 A4 20030730; JP 2002517260 A 20020618
DOCDB simple family (application)
US 9913295 W 19990611; EP 99927501 A 19990611; JP 2000553622 A 19990611