Global Patent Index - EP 1086244 A1

EP 1086244 A1 2001-03-28 - RESTRICTION ENZYME GENE DISCOVERY METHOD

Title (en)

RESTRICTION ENZYME GENE DISCOVERY METHOD

Title (de)

METHODE ZUR ERKENNUNG DER GENE VON RESTRIKTIONSENZYMEN

Title (fr)

DECOUVERTE DE GENES D'ENZYMES DE RESTRICTION

Publication

EP 1086244 A1 (EN)

Application

EP 99927501 A

Priority

  • US 9913295 W
  • US 8908698 P
  • US 8910198 P

Abstract (en)

[origin: WO9964632A1] The invention is directed to direct cloning of intact genes, with a high probability that the orientation of expression is known in advance, and with a low probability of being associated with extraneous possibly toxic genes. The invention is particularly directed to obtaining genes encoded in DNA cassettes comprised of repeat sequences flanking variable open reading frames. The invention encompasses obtaining such cassette-encoded genes using oligonucleotides hybridizing to the repeated elements, cloning them and expressing them. Expression may employ tightly regulated vectors and useful strains disclosed. Methods for identifying restriction endonuclease and DNA methyltransferase genes in the absence of prior information about the sequences or biochemical specificities of these are also disclosed.

IPC 1-7 (main, further and additional classification)

C12Q 1/68; C07H 21/04

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12Q 1/68 (2006.01)

CPC (invention and additional information)

C12N 15/10 (2013.01); C12N 15/1034 (2013.01)

Designated contracting state (EPC)

DE FR GB

EPO simple patent family

WO 9964632 A1 19991216; EP 1086244 A1 20010328; EP 1086244 A4 20030730; JP 2002517260 A 20020618

INPADOC legal status


2007-02-14 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20060817

2004-02-04 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20031217

2003-07-30 [A4] SUPPLEMENTARY SEARCH REPORT

- Effective date: 20030610

2001-03-28 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20010110

2001-03-28 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): DE FR GB