Global Patent Index - EP 1086250 A2

EP 1086250 A2 2001-03-28 - METHOD OF GENERATING NUCLEIC ACID HYBRIDS FOR MUTATION ANALYSIS

Title (en)

METHOD OF GENERATING NUCLEIC ACID HYBRIDS FOR MUTATION ANALYSIS

Title (de)

VERFAHREN ZUR HERSTELLUNG VON NUKLEINSÄUREHYBRIDEN ZUR MUTATIONSANALYSE

Title (fr)

Procédé pour générer des hybrides d'acides nucléiques pour l'analyse de mutations

Publication

EP 1086250 A2 (EN)

Application

EP 99939503 A

Priority

  • GB 9901691 W
  • GB 9812674 A

Abstract (en)

[origin: WO9964624A2] A method of producing a hybrid DNA molecule allowing the assembly of sequences x1, x2.....xn where n is greater than or equal to 3 (e.g. give sequences) from diverse locations into a hybrid molecule for the purpose of mutation analysis. The method comprising the steps of: (1) providing in a single reaction mixture: (a) the sequences x1, x2.......xn and their complementary sequences x1', x2'.........xn', to be assembled into the hybrid molecule; (b) for each pair of complementary sequences defined in (a) a respective pair of PCR primers each having a priming sequence and which are such that the primers hybridising to the 3' ends of any two sequences (xi, x'(i+1)), where i is 1 to (n-1), have specifically complementary linker sequences; (2) effecting a first stage PCR reaction in which those primers provided with linker sequences are present in limiting concentrations; and (3) effecting a second stage PCR reaction using a single pair of primers one of which provides the 5'-end of the sense strand and other of which provides the 3'-end of the anti-sense strand of the required hybrid molecule; whereby said hybrid molecule is generated.

IPC 1-7 (main, further and additional classification)

C12Q 1/68

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12Q 1/68 (2006.01)

CPC (invention and additional information)

C12Q 1/6853 (2013.01); C12N 15/1093 (2013.01); C12Q 1/6811 (2013.01)

Combination set (CPC)

C12Q 1/6853 + C12Q 2549/119 + C12Q 2525/161

Citation (search report)

See references of WO 9964624A3

Designated contracting state (EPC)

BE CH DE DK ES FI FR GB IE IT LI NL SE

EPO simple patent family

WO 9964624 A2 19991216; WO 9964624 A3 20000914; AU 5377599 A 19991230; CA 2330252 A1 19991216; EP 1086250 A2 20010328; GB 9812674 D0 19980812; JP 2002517258 A 20020618

INPADOC legal status


2005-03-09 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20040907

2002-03-13 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20020125

2001-03-28 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20010110

2001-03-28 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): BE CH DE DK ES FI FR GB IE IT LI NL SE