EP 1088073 A2 20010404 - CRYPTIC REGULATORY ELEMENTS OBTAINED FROM PLANTS
Title (en)
CRYPTIC REGULATORY ELEMENTS OBTAINED FROM PLANTS
Title (de)
KRYPTISCHE REGULATIONSELEMENTE AUS PFLANZEN
Title (fr)
ELEMENTS REGULATEURS CRYPTIQUES OBTENUS A PARTIR DE PLANTES
Publication
Application
Priority
- CA 9900578 W 19990622
- CA 2246892 A 19980909
- US 10231298 A 19980622
Abstract (en)
[origin: CA2246892A1] T-DNA tagging with a promoterless .beta.-glucuronidase (GUS) gene generated transgenic Nicotiana tabacum plants that expressed GUS activity either only in developing seed coats, or constitutively. Cloning and deletion analysis of the GUS fusion revealed that the promoter responsible for seed coat specificity was located in the plant DNA proximal to the GUS gene. Analysis of the region demonstrated that the seed coat-specificity of GUS expression in this transgenic plant resulted from T-DNA insertion next to a cryptic promoter. This promoter is useful in controlling the expression of genes to the developing seed coat in plant seeds. Similarly, cloning and characterization of the cryptic constitutive promoter revealed the occurrence of several cryptic regulatory regions. These regions include promoter, negative regulatory elements, transcriptional enhancers, core promoter regions, and translational enhancers and other regulatory elements.
IPC 1-7
IPC 8 full level
A01H 5/00 (2006.01); A01H 5/10 (2006.01); C12N 5/04 (2006.01); C12N 5/10 (2006.01); C12N 15/29 (2006.01); C12N 15/82 (2006.01)
Citation (search report)
See references of WO 9967389A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
AU 4355199 A 20000110; CA 2246892 A1 19991222; CA 2331842 A1 19991229; CA 2331842 C 20071218; EP 1088073 A2 20010404
DOCDB simple family (application)
AU 4355199 A 19990622; CA 2246892 A 19980909; CA 2331842 A 19990622; EP 99926205 A 19990622