Global Patent Index - EP 1088073 A2

EP 1088073 A2 2001-04-04 - CRYPTIC REGULATORY ELEMENTS OBTAINED FROM PLANTS

Title (en)

CRYPTIC REGULATORY ELEMENTS OBTAINED FROM PLANTS

Title (de)

KRYPTISCHE REGULATIONSELEMENTE AUS PFLANZEN

Title (fr)

ELEMENTS REGULATEURS CRYPTIQUES OBTENUS A PARTIR DE PLANTES

Publication

EP 1088073 A2 (EN)

Application

EP 99926205 A

Priority

  • CA 9900578 W
  • CA 2246892 A
  • US 10231298 A

Abstract (en)

[origin: CA2246892A1] T-DNA tagging with a promoterless .beta.-glucuronidase (GUS) gene generated transgenic Nicotiana tabacum plants that expressed GUS activity either only in developing seed coats, or constitutively. Cloning and deletion analysis of the GUS fusion revealed that the promoter responsible for seed coat specificity was located in the plant DNA proximal to the GUS gene. Analysis of the region demonstrated that the seed coat-specificity of GUS expression in this transgenic plant resulted from T-DNA insertion next to a cryptic promoter. This promoter is useful in controlling the expression of genes to the developing seed coat in plant seeds. Similarly, cloning and characterization of the cryptic constitutive promoter revealed the occurrence of several cryptic regulatory regions. These regions include promoter, negative regulatory elements, transcriptional enhancers, core promoter regions, and translational enhancers and other regulatory elements.

IPC 1-7 (main, further and additional classification)

C12N 15/29; A01H 5/00; A01H 5/10; C12N 5/10; C12N 15/82

IPC 8 full level (invention and additional information)

A01H 5/00 (2006.01); A01H 5/10 (2006.01); C12N 5/04 (2006.01); C12N 5/10 (2006.01); C12N 15/29 (2006.01); C12N 15/82 (2006.01)

Citation (search report)

See references of WO 9967389A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

AU 4355199 A 20000110; CA 2246892 A1 19991222; CA 2331842 A1 19991229; CA 2331842 C 20071218; EP 1088073 A2 20010404

INPADOC legal status


2004-12-15 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20040529

2004-01-07 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20031118

2001-04-04 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20010119

2001-04-04 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE