EP 1088108 A1 20010404 - CELL-BASED ASSAY FOR IMMUNODEFICIENCY VIRUS INFECTIVITY AND SENSITIVITY
Title (en)
CELL-BASED ASSAY FOR IMMUNODEFICIENCY VIRUS INFECTIVITY AND SENSITIVITY
Title (de)
AUF ZELLEN BASIERENDER NACHWEIS ZUR INFEKTIÖSITÄT UND SENSIBILITÄT DES IMMUNSCHWÄCHE VIRUS
Title (fr)
ANALYSE A BASE DE CELLULES POUR DETERMINER L'INFECTIVITE ET LA SENSIBILITE DU VIRUS VIH
Publication
Application
Priority
- US 9914104 W 19990623
- US 9031798 P 19980623
Abstract (en)
[origin: WO9967429A1] Methods and reagents for the capture of primary HIV are provided. A cell line expressing CCR5, CXCR4 and CD4 receptors binds and is infected by primary HIV. The cell line contains a marker gene sequence, the marker gene sequence expressed in near linear quantities over at least two orders of magnitude in response to HIV infection. Primary HIV is amplified to create a primary virus stock through insertion of an amplicon gene into the receptor expressing cell line. HIV amplification occurs rapidly and is operative with noninfectious HIV through amplification in the presence of an infectivity complement. The present invention is useful in determining host HIV titer, drug sensitivity, HIV amplification, gene sequencing and co-receptor utilization.
IPC 1-7
IPC 8 full level
G01N 33/569 (2006.01)
CPC (source: EP)
G01N 33/56988 (2013.01)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9967429 A1 19991229; AU 4827899 A 20000110; CA 2331760 A1 19991229; EP 1088108 A1 20010404; EP 1088108 A4 20020417
DOCDB simple family (application)
US 9914104 W 19990623; AU 4827899 A 19990623; CA 2331760 A 19990623; EP 99931859 A 19990623