Global Patent Index - EP 1088108 A1

EP 1088108 A1 2001-04-04 - CELL-BASED ASSAY FOR IMMUNODEFICIENCY VIRUS INFECTIVITY AND SENSITIVITY

Title (en)

CELL-BASED ASSAY FOR IMMUNODEFICIENCY VIRUS INFECTIVITY AND SENSITIVITY

Title (de)

AUF ZELLEN BASIERENDER NACHWEIS ZUR INFEKTIÖSITÄT UND SENSIBILITÄT DES IMMUNSCHWÄCHE VIRUS

Title (fr)

ANALYSE A BASE DE CELLULES POUR DETERMINER L'INFECTIVITE ET LA SENSIBILITE DU VIRUS VIH

Publication

EP 1088108 A1 (EN)

Application

EP 99931859 A

Priority

  • US 9914104 W
  • US 9031798 P

Abstract (en)

[origin: WO9967429A1] Methods and reagents for the capture of primary HIV are provided. A cell line expressing CCR5, CXCR4 and CD4 receptors binds and is infected by primary HIV. The cell line contains a marker gene sequence, the marker gene sequence expressed in near linear quantities over at least two orders of magnitude in response to HIV infection. Primary HIV is amplified to create a primary virus stock through insertion of an amplicon gene into the receptor expressing cell line. HIV amplification occurs rapidly and is operative with noninfectious HIV through amplification in the presence of an infectivity complement. The present invention is useful in determining host HIV titer, drug sensitivity, HIV amplification, gene sequencing and co-receptor utilization.

IPC 1-7 (main, further and additional classification)

C12Q 1/70; C12N 5/10; G01N 33/567

IPC 8 full level (invention and additional information)

G01N 33/569 (2006.01)

CPC (invention and additional information)

G01N 33/56988 (2013.01)

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9967429 A1 19991229; AU 4827899 A 20000110; CA 2331760 A1 19991229; EP 1088108 A1 20010404; EP 1088108 A4 20020417

INPADOC legal status


2004-10-06 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20040328

2004-01-07 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20031117

2002-04-17 [A4] SUPPLEMENTARY SEARCH REPORT

- Effective date: 20020306

2002-04-17 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A4

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2001-04-04 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20001222

2001-04-04 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2001-04-04 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO

- Free text: AL PAYMENT 20001222;LT PAYMENT 20001222;LV PAYMENT 20001222;MK PAYMENT 20001222;RO PAYMENT 20001222;SI PAYMENT 20001222