EP 1090139 A1 20010411 - PH-INDICATOR BASED ASSAY FOR SELECTIVE ENZYMES
Title (en)
PH-INDICATOR BASED ASSAY FOR SELECTIVE ENZYMES
Title (de)
VERFAHREN ZUM QUANTITATIVEN "SCREENING" VON HYDROLASE-"LIBRARIES" UNTER VERWENDUNG VON PH-INDIKATOREN
Title (fr)
ANALYSE ENZYMATIQUE SELECTIVE UTILISANT UN INDICATEUR DE PH
Publication
Application
Priority
- US 9914448 W 19990625
- US 9056998 P 19980625
Abstract (en)
[origin: WO9967420A1] Provided herein is a method for the quantitative screening of hydrolase for desired substrate activity using pH indicators which are sensitive to the release of protons from a chemical reaction in a reaction mixture. The method comprises selecting buffer and indicator conditions such that both have the same affinity for protons such that the relative amount of buffer protonated is proportional to the amount of indicator protonated as the pH of the reaction mixture shifts. A reaction mixture is then prepared comprising a buffer, indicator, hydrolase to be tested, and desired substrate to be tested, allowing the hydrolase to react with the substrate. The reaction is monitored by detection of change in color of the reaction mixture, determined by the affect of the reaction on the indicator.
IPC 1-7
IPC 8 full level
C12Q 1/34 (2006.01)
CPC (source: EP)
C12Q 1/34 (2013.01); C12Q 2334/10 (2013.01)
Citation (search report)
See references of WO 9967420A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9967420 A1 19991229; AU 4833499 A 20000110; EP 1090139 A1 20010411; JP 2004513601 A 20040513
DOCDB simple family (application)
US 9914448 W 19990625; AU 4833499 A 19990625; EP 99931923 A 19990625; JP 2000556060 A 19990625