Global Patent Index - EP 1090139 A1

EP 1090139 A1 2001-04-11 - PH-INDICATOR BASED ASSAY FOR SELECTIVE ENZYMES

Title (en)

PH-INDICATOR BASED ASSAY FOR SELECTIVE ENZYMES

Title (de)

VERFAHREN ZUM QUANTITATIVEN "SCREENING" VON HYDROLASE-"LIBRARIES" UNTER VERWENDUNG VON PH-INDIKATOREN

Title (fr)

ANALYSE ENZYMATIQUE SELECTIVE UTILISANT UN INDICATEUR DE PH

Publication

EP 1090139 A1 (EN)

Application

EP 99931923 A

Priority

  • US 9914448 W
  • US 9056998 P

Abstract (en)

[origin: WO9967420A1] Provided herein is a method for the quantitative screening of hydrolase for desired substrate activity using pH indicators which are sensitive to the release of protons from a chemical reaction in a reaction mixture. The method comprises selecting buffer and indicator conditions such that both have the same affinity for protons such that the relative amount of buffer protonated is proportional to the amount of indicator protonated as the pH of the reaction mixture shifts. A reaction mixture is then prepared comprising a buffer, indicator, hydrolase to be tested, and desired substrate to be tested, allowing the hydrolase to react with the substrate. The reaction is monitored by detection of change in color of the reaction mixture, determined by the affect of the reaction on the indicator.

IPC 1-7 (main, further and additional classification)

C12Q 1/34

IPC 8 full level (invention and additional information)

C12Q 1/34 (2006.01)

CPC (invention and additional information)

C12Q 1/34 (2013.01); C12Q 2334/10 (2013.01)

Citation (search report)

See references of WO 9967420A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9967420 A1 19991229; AU 4833499 A 20000110; EP 1090139 A1 20010411; JP 2004513601 A 20040513

INPADOC legal status


2004-07-14 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20040103

2003-04-09 [RAP1] TRANSFER OF RIGHTS OF AN EP PUBLISHED APPLICATION

- Owner name: MCGILL UNIVERSITY

2001-07-04 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20010521

2001-04-11 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20010122

2001-04-11 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2001-04-11 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO

- Free text: AL PAYMENT 20010122;LT PAYMENT 20010122;LV PAYMENT 20010122;MK PAYMENT 20010122;RO PAYMENT 20010122;SI PAYMENT 20010122