Global Patent Index - EP 1090140 A1

EP 1090140 A1 2001-04-11 - METHOD FOR SCREENING FOR SUBSTANCES WHICH ARE ACTIVATORS OR INHIBITORS OF PROTEIN KINASE B

Title (en)

METHOD FOR SCREENING FOR SUBSTANCES WHICH ARE ACTIVATORS OR INHIBITORS OF PROTEIN KINASE B

Title (de)

VERFAHREN UND SCREENING NACH SUBSTANZEN, DIE AKTIVATOREN ODER INHIBITOREN VON PROTEINKINASE B SIND

Title (fr)

PROCEDE DETECTION DE SUBSTANCES ACTIVATRICES ET INHIBITRICES DE LA PROTEINE KINASE B

Publication

EP 1090140 A1 (EN)

Application

EP 99948555 A

Priority

  • SE 9900609 W
  • SE 9801530 A

Abstract (en)

[origin: WO9957305A1] The present invention relates to methods for screening for substances which are activators or inhibitors of Protein kinase B (PKB) and can be used as a kinase substrate for PKB by the use of peptides comprising a specific sequence which do not include a large hydrophobic residue at the C-terminal end. These peptides can be used in assays measuring the activity of PKB, in screening for substances which are activators or inhibitors of gene transcriptional regulation of forkhead proteins through the catalytic activities of PKB and for discrimination between the effects of compounds which mediate insulin action through transcription from those which modulate activity of enzymes involved in metabolism by phosphorylation. The substrate peptide sequence comprises the sequence 1 ArgXaaArgXaaXaaSerXaa or sequence 2, in which, Xaa in position 2 is any amino acid, preferably chosen from Pro and Gly, Xaa in positions 4 and 5 are any amino acid, preferably chosen from Thr and Ser and Xaa in position 7 is any amino acid, preferably chosen from Asn, Gln, Thr, Ser and with the proviso that the sequence does not include a large hydrophobic residue directly C-terminal to the phosphorylation site.

IPC 1-7 (main, further and additional classification)

C12Q 1/48

IPC 8 full level (invention and additional information)

C12N 9/99 (2006.01); C07K 7/06 (2006.01); C12Q 1/48 (2006.01)

CPC (invention and additional information)

C07K 7/06 (2013.01); C12Q 1/48 (2013.01); G01N 2333/9121 (2013.01); G01N 2500/20 (2013.01)

Citation (search report)

See references of WO 9957305A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9957305 A1 19991111; AU 4298299 A 19991123; AU 754508 B2 20021121; CA 2327540 A1 19991111; EP 1090140 A1 20010411; JP 2002514389 A 20020521; SE 9801530 D0 19980430

INPADOC legal status


2004-03-31 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20030923

2003-06-25 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20030512

2001-11-14 [RAP1] TRANSFER OF RIGHTS OF AN EP PUBLISHED APPLICATION

- Owner name: BIOVITRUM AB

2001-04-11 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20001106

2001-04-11 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE