EP 1092046 A4 20030219 - METHODS, COMPOSITIONS AND APPARATUS FOR MAKING NUCLEIC ACID MOLECULES HAVING A SELECTED AFFINITY TO A TARGET MOLECULE
Title (en)
METHODS, COMPOSITIONS AND APPARATUS FOR MAKING NUCLEIC ACID MOLECULES HAVING A SELECTED AFFINITY TO A TARGET MOLECULE
Title (de)
VERFAHREN, ZUSAMMENSETZNGEN UND APPARAT ZUR HERSTELLUNG VON NUKLEINSÄUREMOLEKÜLEN MIT SELEKTIVER AFFINITÄT FÜR EIN ZIELMOLEKÜL
Title (fr)
PROCEDES, COMPOSITIONS ET DISPOSITIF DE FABRICATION D'ACIDES NUCLEIQUES PRESENTANT UNE AFFINITE SELECTIVE POUR UNE MOLECULE CIBLE
Publication
Application
Priority
- US 9915030 W 19990701
- US 9157898 P 19980702
Abstract (en)
[origin: WO0001849A1] The present invention is directed to methods, compositions, kits and devices for making a nucleic acid having selected affinity to a target molecule. One embodiment of the present invention is a method of making a replicatable nucleic acid template having a selected affinity to a target. The method comprises the step of applying a selection to a first generation comprising at least one replicatable nucleic acid template as the replicatable nucleic acid template is replicated by a nucleic acid polymerase to form at least one subsequent generation comprising a replicatable nucleic acid template. The selection is based on the affinity of the replicatable nucleic template of different generations to the target. The nucleic acid polymerase introduces genetic variability between generations of the replicatable nucleic acid template to produce replicatable nucleic acid templates having different affinities to the target. The replicatable nucleic acid templates are separated on the basis of the affinity of the replicatable nucleic acid template to the target.
IPC 1-7
IPC 8 full level
C12M 1/34 (2006.01); C12N 15/10 (2006.01); C12P 19/34 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP US)
C12N 15/1058 (2013.01 - EP US)
Citation (search report)
- [A] US 5567588 A 19961022 - GOLD LARRY [US], et al
- [T] WO 9958661 A1 19991118 - DIATECH PTY LTD [AU], et al
- [T] WO 9854312 A1 19981203 - BABRAHAM INST [GB], et al
- [PA] WO 9836100 A1 19980820 - INVITRO DIAGNOSTICS INC [US], et al
- [XY] ELLINGTON A D ET AL: "IN VITRO SELECTION OF RNA MOLECULES THAT BIND SPECIFIC LIGANDS", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 346, 30 August 1990 (1990-08-30), pages 818 - 822, XP000673540, ISSN: 0028-0836
- [XY] SASSANFAR MANDANA ET AL: "An RNA motif that binds ATP", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 364, no. 6437, 5 August 1993 (1993-08-05), pages 550 - 553, XP002204732, ISSN: 0028-0836
- [Y] BIEBRICHER C K: "REPLICATION AND EVOLUTION OF SHORT-CHAINED RNA SPECIES REPLICATED BY QBETA REPLICASE", COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, NEW YORK, NY, US, vol. 52, 1987, pages 299 - 306, XP000984949
- [Y] HANES J & PLÜCKTHUN A: "In vitro selection and evolution of functional proteins by using ribosome display", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, no. 94, pages 4937 - 4942, XP002077149, ISSN: 0027-8424
- [PX] COX J C ET AL: "AUTOMATED RNA SELECTION", BIOTECHNOLOGY PROGRESS, vol. 14, October 1998 (1998-10-01), pages 845 - 850, XP002927340, ISSN: 8756-7938
- [A] BURKE J M ET AL: "IN VITRO SELECTION AND EVOLUTION OF RNA: APPLICATIONS FOR CATALYTIC RNA, MOLECULAR RECOGNITION, AND DRUG DISCOVERY", FASEB JOURNAL, FED. OF AMERICAN SOC. FOR EXPERIMENTAL BIOLOGY, BETHESDA, MD, US, vol. 7, no. 1, 1993, pages 106 - 112, XP000567892, ISSN: 0892-6638
- [A] BIEBRICHER C K ET AL: "MOLECULAR EVOLUTION OF RNA IN VITRO", BIOPHYSICAL CHEMISTRY, AMSTERDAM, NL, vol. 66, no. 2/3, 30 June 1997 (1997-06-30), pages 179 - 192, XP000989587
- [A] FAMULOK M ET AL: "IN VITRO SELECTION OF SPECIFIC LIGAND-BINDING NUCLEIC ACIDS**", ANGEWANDTE CHEMIE. INTERNATIONAL EDITION, VERLAG CHEMIE. WEINHEIM, DE, vol. 31, no. 8, 1 August 1992 (1992-08-01), pages 979 - 988, XP000298700, ISSN: 0570-0833
- [A] JOYCE GERALD F: "In vitro evolution of nucleic acids.", CURRENT OPINION IN STRUCTURAL BIOLOGY, vol. 4, no. 3, 1994, pages 331 - 336, XP001120621, ISSN: 0959-440X
- [A] TSANG J ET AL: "In vitro evolution of randomized ribozymes.", METHODS IN ENZYMOLOGY. UNITED STATES 1996, vol. 267, 1996, pages 410 - 426, XP001120470, ISSN: 0076-6879
- [A] TUERK CRAIG ET AL: "In vitro evolution of functional nucleic acids: High-affinity RNA ligands of the HIV-1 rev protein.", PCR: THE POLYMERASE CHAIN REACTION., 1994, Birkhaeuser Boston, Inc.; Birkhaeuser Verlag 175 Fifth Avenue, New York, New York 10010, USA; P. O. Box 133, CH-4010 Basel, Switzerland, pages 233 - 243, XP001120462, ISBN: 0-8176-3607-2
- See references of WO 0001849A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0001849 A1 20000113; AU 4854999 A 20000124; EP 1092046 A1 20010418; EP 1092046 A4 20030219; US 2003087233 A1 20030508; US 2005272081 A1 20051208
DOCDB simple family (application)
US 9915030 W 19990701; AU 4854999 A 19990701; EP 99932187 A 19990701; US 13567305 A 20050524; US 75223100 A 20001229