Global Patent Index - EP 1095163 A2

EP 1095163 A2 2001-05-02 - SCREENING OF HYDROLASE LIBRARIES FOR ENANTIOSELECTIVE ENZYMES

Title (en)

SCREENING OF HYDROLASE LIBRARIES FOR ENANTIOSELECTIVE ENZYMES

Title (de)

SIEBUNG DER HYDROLASEBIBLIOTHEKEN FÜR ENANTIOSELECTIVE ENZYME

Title (fr)

VISUALISATION DE REACTIONS CATALYSEES PAR DES ENZYMES UTILISANT DES INDICATEURS DE PH: CRIBLAGE RAPIDE DE BIBLIOTHEQUES D'HYDROLASES POUR DETECTER DES ENZYMES ENANTIOSELECTIVES

Publication

EP 1095163 A2 (EN)

Application

EP 99935446 A

Priority

  • US 9915400 W
  • US 9188098 P
  • US 12570899 P

Abstract (en)

[origin: WO0001842A2] The use of pH indicators to monitor enzyme-catalyzed reactions is described. The formation of acid following an enzyme-mediated reaction, such as hydrolysis, causes a drop in the pH that can be visualized by a change in the color of the indicator-containing solution. Preferred indicators are those showing a color transition within the operational pH range of the enzyme. Using the present system, the enantioselectivity of enzymes such as lipases and esterases can be estimated using single isomers under the same conditions and comparing the color turnover for each one. The method is also useful for application to the hierarchical screening of a library of enzymes.

IPC 1-7 (main, further and additional classification)

C12Q 1/34; C12N 15/12; C12Q 1/37; C12Q 1/44

IPC 8 full level (invention and additional information)

C12N 9/18 (2006.01); C12N 15/10 (2006.01); C12Q 1/34 (2006.01); C12Q 1/37 (2006.01); C12Q 1/44 (2006.01)

CPC (invention and additional information)

C12N 15/1034 (2013.01); C12N 9/18 (2013.01); C12Q 1/34 (2013.01); C12Q 1/37 (2013.01); C12Q 1/44 (2013.01)

Citation (search report)

See references of WO 0001842A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 0001842 A2 20000113; WO 0001842 A3 20000504; WO 0001842 A8 20000608; AU 5092499 A 20000124; CA 2332638 A1 20000113; EP 1095163 A2 20010502

INPADOC legal status


2003-08-13 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20030201

2001-05-02 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20010206

2001-05-02 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2001-05-02 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO

- Free text: AL PAYMENT 20010206;LT PAYMENT 20010206;LV PAYMENT 20010206;MK PAYMENT 20010206;RO PAYMENT 20010206;SI PAYMENT 20010206