Global Patent Index - EP 1095256 A2

EP 1095256 A2 2001-05-02 - HIGH NUMERICAL APERTURE FLOW CYTOMETER AND METHOD OF USING SAME

Title (en)

HIGH NUMERICAL APERTURE FLOW CYTOMETER AND METHOD OF USING SAME

Title (de)

VERFAHREN UND DURCHFLUSSZYTOMETER MIT HOHER NUMERISCHER APERTUR

Title (fr)

CYTOMETRE EN FLUX A OUVERTURE NUMERIQUE ELEVEE ET PROCEDE DE SON UTILISATION

Publication

EP 1095256 A2 (EN)

Application

EP 00914609 A

Priority

  • US 0004069 W
  • US 12080499 P

Abstract (en)

[origin: WO0049387A2] The high numerical aperture flow cytometer of the present invention includes a flow cell and a laser input. The laser input emits a beam of light that is oriented substantially orthoganilly to the flow of blood cells through the flow cell such that laser light impinges upon the blood cells as they pass through the flow cell. A portion of the beam from the laser input that impinges upon the blood cells in the flow cell is scattered at a substantially right angle to the beam of laser input ("right angle scatter"). A second portion of the beam from the laser input that impinges upon the cells in the flow cell is scattered at a much lower angle than 90 DEG . This scatter is termed "low angle forward scatter light" and has an angle of from about 2 DEG to about 5 DEG from the orientation of the original beam from laser input. A right angle scatter light detector is oriented to receive the previously mentioned right angle scatter light. A low angle forward scatter light detector is oriented to capture the previously mentioned low angled forward scatter light oriented at about 2 DEG to about 5 DEG beam from laser input.

IPC 1-7 (main, further and additional classification)

G01N 15/14

IPC 8 full level (invention and additional information)

G01N 15/14 (2006.01); G01N 15/00 (2006.01)

CPC (invention and additional information)

G01N 15/14 (2013.01); G01N 2015/008 (2013.01); G01N 2015/1447 (2013.01)

Citation (search report)

See references of WO 0049387A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 0049387 A2 20000824; WO 0049387 A3 20010215; WO 0049387 A9 20010927; AU 3597600 A 20000904; AU 768616 B2 20031218; AU 768616 C 20041216; CA 2329031 A1 20000824; CA 2329031 C 20030923; EP 1095256 A2 20010502; JP 2002537557 A 20021105

INPADOC legal status


2007-06-20 [18R] REFUSED

- Effective date: 20070116

2001-05-02 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20001024

2001-05-02 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE