Global Patent Index - EP 1095267 A1

EP 1095267 A1 2001-05-02 - METHOD TO DETECT AND ANALYZE TIGHT-BINDING LIGANDS IN COMPLEX BIOLOGICAL SAMPLES

Title (en)

METHOD TO DETECT AND ANALYZE TIGHT-BINDING LIGANDS IN COMPLEX BIOLOGICAL SAMPLES

Title (de)

VERFAHREN ZUR FESTSTELLUNG UND ANALYSE VON STARK BINDENDEN LIGANDEN IN KOMPLEXEN BIOLOGISCHEN PROBEN

Title (fr)

PROCEDE DE DETECTION ET D'ANALYSE DE LIGANDS A FORTE FIXATION DANS DES ECHANTILLONS BIOLOGIQUES COMPLEXES

Publication

EP 1095267 A1 (EN)

Application

EP 99933790 A

Priority

  • US 9915458 W
  • US 9240398 P
  • US 9429798 P
  • US 9827463 W

Abstract (en)

[origin: WO0003240A1] The invention combines a capillary electrophoresis (CE) technique for screening complex biological samples with mass spectrometry (MS), to provide a streamlined procedure for identifying and characterizing candidate ligands in a complex biological sample that bind at a selected binding strength to a selected target molecule. The method of the invention advantageously identifies and characterizes tight-binding ligands when high concentrations of weak ligands are present in the sample, which may mask lower concentrations of tight-binding ligands in the sample. The method involves interfacing a capillary from a CE instrument with a post-capillary mass spectrometer to provide direct mass analysis of target/ligand complexes that migrate stably through the CE instrument. All weaker-binding ligands will not be detected during the MS analysis because they dissociate from the target early during the CE run, before reaching and entering the mass spectrometer. Therefore, this method can identify and structurally characterize moderate-to-tight-binding ligands in complex biological samples, even in the presence of high concentrations of weak-binding ligands.

IPC 1-7 (main, further and additional classification)

G01N 27/447

IPC 8 full level (invention and additional information)

G01N 27/62 (2006.01); G01N 27/447 (2006.01); G01N 33/561 (2006.01)

CPC (invention and additional information)

G01N 27/44726 (2013.01); G01N 27/44717 (2013.01); G01N 33/561 (2013.01)

Citation (search report)

See references of WO 0003240A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 0003240 A1 20000120; EP 1095267 A1 20010502; JP 2004500541 A 20040108

INPADOC legal status


2008-07-23 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20080201

2001-07-04 [RAP1] TRANSFER OF RIGHTS OF AN EP PUBLISHED APPLICATION

- Owner name: CETEK CORPORATION

2001-05-02 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20010212

2001-05-02 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE