Global Patent Index - EP 1097217 A2

EP 1097217 A2 2001-05-09 - METABOLICALLY ENGINEERED MICROBIAL CELL COMPRISING A MODIFIED REDOX ACTIVITY

Title (en)

METABOLICALLY ENGINEERED MICROBIAL CELL COMPRISING A MODIFIED REDOX ACTIVITY

Title (de)

IN IHREM STOFFWECHSEL VERÄNDERTE MIKROBIELLE ZELLE DIE EINE VERÄNDERTE REDOXAKTIVITÄT ENTHÄLT

Title (fr)

CELLULE MICROBIENNE PRODUITE PAR INGENIERIE METABOLIQUE ET POSSEDANT UNE ACTIVITE OXYDOREDUCTRICE

Publication

EP 1097217 A2 (EN)

Application

EP 99932683 A

Priority

  • DK 9900398 W
  • DK PA199800968 A

Abstract (en)

[origin: WO0003021A2] The present invention concerns a microbial cell comprising a first expressible enzyme activity, e.g., pyridine nucleotide transhydrogenase from Azotobacter vinelandii which, when expressed in said microbial cell, is controlling an intracellular redox system of said cell, said first expressible enzyme activity in said microbial cell being operably linked to an expression signal not natively associated with said first expressible enzyme activity. The expression of said first expressible enzyme activity is operably linked to an increased production of a first metabolite. The cell is preferably a yeast cell and may also comprise a further expressible enzyme activity, e.g., glutamate synthase and/or glutamine synthetase, said further expressible enzyme activity, when expressed, mediating a first biosynthetic reaction resulting in production of a first metabolite, said further expressible enzyme activity, when expressed at an increased level, resulting in an increased production of said first metabolite, said increased expression of said further expressible enzyme activity and/or said increased production of said first metabolite being operably linked to an increased expression of said first expressible enzyme activity. There is also provided a microbial cell wherein said expression of said first expressible enzyme activity is operably linked to an increased production of a first metabolite and a decreased production of a second metabolite. Cells according to the invention are useful in the production of a metabolite such as ethanol or glycerol.

IPC 1-7 (main, further and additional classification)

C12N 15/52; C12N 1/19; C12N 9/00; C12N 9/02; C12N 9/06; C12N 15/53; C12P 7/06; C12P 7/20

IPC 8 full level (invention and additional information)

C12N 1/18 (2006.01); C12N 1/20 (2006.01); C12N 9/00 (2006.01); C12N 9/02 (2006.01); C12N 9/06 (2006.01); C12P 1/00 (2006.01); C12P 7/06 (2006.01); C12P 7/20 (2006.01); C12P 7/56 (2006.01)

CPC (invention and additional information)

C12N 9/0036 (2013.01); C12N 1/18 (2013.01); C12N 1/20 (2013.01); C12N 9/0016 (2013.01); C12N 9/93 (2013.01); C12P 1/00 (2013.01); C12P 7/06 (2013.01); C12P 7/20 (2013.01); C12P 7/56 (2013.01); C12R 1/865 (2013.01); Y02E 50/17 (2013.01)

Citation (search report)

See references of WO 0003021A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 0003021 A2 20000120; WO 0003021 A3 20000420; AU 4897999 A 20000201; CA 2379034 A1 20000120; EP 1097217 A2 20010509

INPADOC legal status


2003-06-25 [18W] WITHDRAWN

- Effective date: 20030422

2002-01-30 [RAP1] TRANSFER OF RIGHTS OF AN EP PUBLISHED APPLICATION

- Owner name: N&N BIOTECH APS

2001-05-09 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20010208

2001-05-09 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2001-05-09 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO

- Free text: AL;LT;LV;MK;RO;SI