Global Patent Index - EP 1097226 A1

EP 1097226 A1 2001-05-09 - USURPIN, A MAMMALIAN DED-CASPASE HOMOLOGUE THAT PRECLUDES CASPASE-8 RECRUITMENT AND ACTIVATION BY THE CD-95 (FAS, APO-1) RECEPTOR COMPLEX

Title (en)

USURPIN, A MAMMALIAN DED-CASPASE HOMOLOGUE THAT PRECLUDES CASPASE-8 RECRUITMENT AND ACTIVATION BY THE CD-95 (FAS, APO-1) RECEPTOR COMPLEX

Title (de)

USURPIN, SÄUGERTIER DED-CASPASE HOMOLOGES, DAS DIE AKTIVIERUNG DER CASPASE-8 BEIM CD-95 (FAS, APO-1) REZEPTOR INHIBIERT

Title (fr)

L'USURPINE, HOMOLOGUE MAMMIFERE DE LA DED-CASPASE, EMPECHANT LE RECRUTEMENT DE LA CASPASE-8 ET L'ACTIVATION DE CELLE-CI PAR LE COMPLEXE DU RECEPTEUR DE CD-95 (FAS, APO-1)

Publication

EP 1097226 A1 (EN)

Application

EP 99927635 A

Priority

  • CA 9900615 W
  • US 9200598 P

Abstract (en)

[origin: WO0003023A1] The protein Usurpin, an endogenous mammalian regulator of the process of apoptotic cell suicide, is provided. Provided are three Usurpin proteins having an amino acid sequence selected from the group consisting of: SEQ.ID.NO.:4, SEQ.ID.NO.:5, and SEQ.ID.NO.:6 that arise from alternative splicing of the Usurpin gene. The full-length Usurpin polypeptide (Usurpin- alpha ) has features in common with pro-caspase-8 and -10, including tandem "death effector domains" (DEDs) on the N-terminus and a large subunit/small subunit caspase-like domain, but it lacks key residues that are necessary for caspase proteolytic activity. However, Usurpin heterodimerizes with pro-caspase-8 and precludes pro-caspase-8 recruitment by the FADD/MORT1 adapter protein, thus blocking an important interaction in the CD95 (Fas/APO-1) apoptotic process. Therefore, Usurpin is useful as a modulator of the sensitivity of cells to CD95 (Fas/APO-1)-mediated apoptosis. Accordingly, the present invention also provides methods of modulating apoptosis by introducing Usurpin polypeptides into cells. Also provided are various purified nucleotide sequences encoding Usurpin. Also provided are methods of identifying inhibitors of the interaction between Usurpin and pro-caspase-8. Such inhibitors will be useful in controlling the interaction between Usurpin and pro-caspase-8, thus modulating apoptosis.

IPC 1-7 (main, further and additional classification)

C12N 15/57; C07K 16/40; C12N 1/21; C12N 5/10; C12N 9/64; G01N 33/50

IPC 8 full level (invention and additional information)

G01N 33/50 (2006.01); C07K 14/47 (2006.01); C07K 16/18 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 9/64 (2006.01); C12N 15/09 (2006.01); C12N 15/57 (2006.01); C12Q 1/02 (2006.01)

CPC (invention and additional information)

C12N 9/6475 (2013.01)

Citation (search report)

See references of WO 0003023A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 0003023 A1 20000120; CA 2332346 A1 20000120; EP 1097226 A1 20010509; JP 2002520025 A 20020709

INPADOC legal status


2004-10-27 [18D] APPLICATION DEEMED TO BE WITHDRAWN

- Effective date: 20040420

2003-11-19 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20031007

2001-05-09 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20010208

2001-05-09 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2001-05-09 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO:

- Free text: AL;LT;LV;MK;RO;SI