EP 1097227 A1 20010509 - CLEAVAGE OF FUSION PROTEINS CONTAINING PART OF A CAULOBACTER S-LAYER PROTEIN

Title (en)

CLEAVAGE OF FUSION PROTEINS CONTAINING PART OF A CAULOBACTER S-LAYER PROTEIN

Title (de)

SPALTUNG VON FUSIONSPROTEINEN, WELCHE EINEN TEIL EINES CAULOBACTER S-LAYER PROTEINS BEINHALTEN

Title (fr)

CLIVAGE DE PROTEINES HYBRIDES CONTENANT UNE PARTIE D'UNE PROTEINE S-LAYER DE CAULOBACTER

Publication

EP 1097227 A1 20010509 (EN)

Application

EP 99928972 A 19990714

Priority

CA 9900637 W 19990714
CA 2237704 A 19980714

Abstract (en)

[origin: WO0004170A1] This invention provides a method for cleaving target proteins from Caulobacter S-layer protein under mild acid conditions. A fusion protein secreted by Caulobacter which includes a target protein and a Caulobacter S-layer secretion signal may be cleaved at an aspartate-proline dipeptide without solubilizing the fusion protein. This method may be carried out while the fusion protein is in an insoluble aggregate which facilitates recovery of the protein. This invention also provides a method of preparing a DNA construct for expression of the fusion protein and a method of preparing the fusion protein.

IPC 1-7

C12N 15/62; C12N 15/74; C07K 14/21; C07K 14/00; C07K 14/08; C07K 14/245

IPC 8 full level

C07K 14/00 (2006.01); C07K 14/08 (2006.01); C07K 14/195 (2006.01); C07K 14/21 (2006.01); C07K 14/245 (2006.01); C07K 19/00 (2006.01); C12N 15/09 (2006.01); C12N 15/62 (2006.01); C12P 21/02 (2006.01)

CPC (source: EP)

C07K 14/001 (2013.01); C07K 14/005 (2013.01); C07K 14/21 (2013.01); C07K 14/245 (2013.01); C12N 15/625 (2013.01); C07K 2319/036 (2013.01); C12N 2720/10022 (2013.01)

Citation (search report)

See references of WO 0004170A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 0004170 A1 20000127; AU 4597399 A 20000207; CA 2237704 A1 20000114; EP 1097227 A1 20010509; JP 2002520060 A 20020709

DOCDB simple family (application)

CA 9900637 W 19990714; AU 4597399 A 19990714; CA 2237704 A 19980714; EP 99928972 A 19990714; JP 2000560267 A 19990714