Global Patent Index - EP 1097227 A1

EP 1097227 A1 2001-05-09 - CLEAVAGE OF FUSION PROTEINS CONTAINING PART OF A CAULOBACTER S-LAYER PROTEIN

Title (en)

CLEAVAGE OF FUSION PROTEINS CONTAINING PART OF A CAULOBACTER S-LAYER PROTEIN

Title (de)

SPALTUNG VON FUSIONSPROTEINEN, WELCHE EINEN TEIL EINES CAULOBACTER S-LAYER PROTEINS BEINHALTEN

Title (fr)

CLIVAGE DE PROTEINES HYBRIDES CONTENANT UNE PARTIE D'UNE PROTEINE S-LAYER DE CAULOBACTER

Publication

EP 1097227 A1 (EN)

Application

EP 99928972 A

Priority

  • CA 9900637 W
  • CA 2237704 A

Abstract (en)

[origin: WO0004170A1] This invention provides a method for cleaving target proteins from <u>Caulobacter</u> S-layer protein under mild acid conditions. A fusion protein secreted by <u>Caulobacter</u> which includes a target protein and a <u>Caulobacter</u> S-layer secretion signal may be cleaved at an aspartate-proline dipeptide without solubilizing the fusion protein. This method may be carried out while the fusion protein is in an insoluble aggregate which facilitates recovery of the protein. This invention also provides a method of preparing a DNA construct for expression of the fusion protein and a method of preparing the fusion protein.

IPC 1-7 (main, further and additional classification)

C12N 15/62; C07K 14/00; C07K 14/08; C07K 14/21; C07K 14/245; C12N 15/74

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); C07K 14/00 (2006.01); C07K 14/08 (2006.01); C07K 14/195 (2006.01); C07K 14/21 (2006.01); C07K 14/245 (2006.01); C07K 19/00 (2006.01); C12N 15/62 (2006.01); C12P 21/02 (2006.01)

CPC (invention and additional information)

C07K 14/005 (2013.01); C07K 14/001 (2013.01); C07K 14/21 (2013.01); C07K 14/245 (2013.01); C12N 15/625 (2013.01); C07K 2319/036 (2013.01); C12N 2720/10022 (2013.01)

Citation (search report)

See references of WO 0004170A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 0004170 A1 20000127; AU 4597399 A 20000207; CA 2237704 A1 20000114; EP 1097227 A1 20010509; JP 2002520060 A 20020709

INPADOC legal status


2005-12-07 [18D] APPLICATION DEEMED TO BE WITHDRAWN

- Effective date: 20050531

2004-08-11 [RAP1] TRANSFER OF RIGHTS OF AN APPLICATION

- Owner name: RESEARCH CORPORATION TECHNOLOGIES, INC.

2003-11-12 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20030924

2003-10-08 [RTI1] TITLE (CORRECTION)

- Free text: CLEAVAGE OF FUSION PROTEINS CONTAINING PART OF A CAULOBACTER S-LAYER PROTEIN

2001-05-09 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20010205

2001-05-09 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2001-05-09 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO:

- Free text: AL;LT;LV;MK;RO;SI