Global Patent Index - EP 1097229 A1

EP 1097229 A1 2001-05-09 - PROCESS TO COLLECT METABOLITES FROM MODIFIED NECTAR BY INSECTS

Title (en)

PROCESS TO COLLECT METABOLITES FROM MODIFIED NECTAR BY INSECTS

Title (de)

VERFAHREN ZUM SAMMELN VON METABOLITEN AUS VERÄNDERTEM NEKTAR DURCH INSEKTEN

Title (fr)

PROCEDE PERMETTANT D'UTILISER DES INSECTES POUR RECUEILLIR DES METABOLITES DANS UN NECTAR MODIFIE

Publication

EP 1097229 A1 (EN)

Application

EP 99935173 A

Priority

  • EP 99935173 A
  • EP 98202375 A
  • EP 98204215 A
  • NL 9900453 W

Abstract (en)

[origin: WO0004176A1] The invention relates to a recombinant double-stranded DNA molecule comprising an expression cassette comprising the following constituents: i) a promoter functional in nectaries of plants, ii) a DNA sequence encoding a protein which is fused to the promoter, iii) a DNA sequence encoding a signal peptide that targets the recombinant protein to nectar, which is translationally fused to the DNA sequence encoding the recombinant protein, and optionally iv) a signal sequence functional in plants for the transcription termination and polyadenylation of an RNA molecule. The invention further relates to a process for producing a recombinant gene product from honey, comprising: i) producing a transgenic plant by introducing in a plant cell a recombinant double-stranded DNA molecule, regenerating plants from the transgenic cell, and selecting modified plants exhibiting excretion of the recombinant gene product in nectar, ii) allowing insects, preferably bees, to collect nectar from the transgenic plants and to process the nectar into honey, and iii) isolating and purifying the gene product from the honey.

IPC 1-7 (main, further and additional classification)

C12N 15/82; A01H 5/00; C07K 14/415; C12N 5/10

IPC 8 full level (invention and additional information)

A01H 5/00 (2006.01); A23L 21/25 (2016.01); C07K 14/415 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 15/09 (2006.01); C12N 15/82 (2006.01)

CPC (invention and additional information)

C12N 15/8257 (2013.01); C07K 14/415 (2013.01); C12N 15/823 (2013.01); C12N 15/8231 (2013.01); C12N 15/8242 (2013.01)

Citation (search report)

See references of WO 0004176A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 0004176 A1 20000127; AU 5070699 A 20000207; AU 772119 B2 20040408; CA 2333897 A1 20000127; EP 1097229 A1 20010509; IL 140911 D0 20020210; JP 2002520064 A 20020709; NZ 509813 A 20040130

INPADOC legal status


2007-08-01 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20070201

2003-08-13 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20030626

2002-01-16 [RAP1] TRANSFER OF RIGHTS OF AN EP PUBLISHED APPLICATION

- Owner name: PLANT RESEARCH INTERNATIONAL B.V.

2001-05-09 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20010116

2001-05-09 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2001-05-09 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO

- Free text: AL;LT;LV;MK;RO;SI