EP 1097229 A1 20010509 - PROCESS TO COLLECT METABOLITES FROM MODIFIED NECTAR BY INSECTS
Title (en)
PROCESS TO COLLECT METABOLITES FROM MODIFIED NECTAR BY INSECTS
Title (de)
VERFAHREN ZUM SAMMELN VON METABOLITEN AUS VERÄNDERTEM NEKTAR DURCH INSEKTEN
Title (fr)
PROCEDE PERMETTANT D'UTILISER DES INSECTES POUR RECUEILLIR DES METABOLITES DANS UN NECTAR MODIFIE
Publication
Application
Priority
- EP 99935173 A 19990715
- EP 98202375 A 19980716
- EP 98204215 A 19981214
- NL 9900453 W 19990715
Abstract (en)
[origin: WO0004176A1] The invention relates to a recombinant double-stranded DNA molecule comprising an expression cassette comprising the following constituents: i) a promoter functional in nectaries of plants, ii) a DNA sequence encoding a protein which is fused to the promoter, iii) a DNA sequence encoding a signal peptide that targets the recombinant protein to nectar, which is translationally fused to the DNA sequence encoding the recombinant protein, and optionally iv) a signal sequence functional in plants for the transcription termination and polyadenylation of an RNA molecule. The invention further relates to a process for producing a recombinant gene product from honey, comprising: i) producing a transgenic plant by introducing in a plant cell a recombinant double-stranded DNA molecule, regenerating plants from the transgenic cell, and selecting modified plants exhibiting excretion of the recombinant gene product in nectar, ii) allowing insects, preferably bees, to collect nectar from the transgenic plants and to process the nectar into honey, and iii) isolating and purifying the gene product from the honey.
IPC 1-7
IPC 8 full level
A01H 5/00 (2006.01); A23L 1/08 (2006.01); A23L 21/25 (2016.01); C07K 14/415 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 15/09 (2006.01); C12N 15/82 (2006.01)
CPC (source: EP)
C07K 14/415 (2013.01); C12N 15/823 (2013.01); C12N 15/8231 (2013.01); C12N 15/8242 (2013.01); C12N 15/8257 (2013.01)
Citation (search report)
See references of WO 0004176A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0004176 A1 20000127; AU 5070699 A 20000207; AU 772119 B2 20040408; CA 2333897 A1 20000127; EP 1097229 A1 20010509; IL 140911 A0 20020210; JP 2002520064 A 20020709; NZ 509813 A 20040130
DOCDB simple family (application)
NL 9900453 W 19990715; AU 5070699 A 19990715; CA 2333897 A 19990715; EP 99935173 A 19990715; IL 14091199 A 19990715; JP 2000560273 A 19990715; NZ 50981399 A 19990715