Global Patent Index - EP 1098006 A1

EP 1098006 A1 20010509 - Simultaneous detection, identification and differentiation of eubacterial taxa using a hybridization assay

Title (en)

Simultaneous detection, identification and differentiation of eubacterial taxa using a hybridization assay

Title (de)

Gleichzeitiger Nachweis, Identifizierung und Differenzierung von Eubakterien unter Verwendung eines Hybridisierungs-Assays

Title (fr)

Detection, identification et differentiation simultanees de taxa d'eubacteriales a l'aide d'une technique d'hybridation

Publication

EP 1098006 A1 20010509 (EN)

Application

EP 01200045 A 19950623

Priority

  • EP 01200045 A 19950623
  • EP 95924923 A 19950623
  • EP 94870106 A 19940624
  • EP 95870032 A 19950407

Abstract (en)

The present invention relates to a method for detection and identification of at least one micro-organism, or for the simultaneous detection of several micro-organisms in a sample, comprising the steps of: (i) if need be releasing, isolating or concentrating the polynucleic acids present in the sample; (ii) if need be amplifying the 16S-23S rRNA spacer region, or a part of it, with at least one suitable primer pair; (iii) hybridizing the polynucleic acids of step (i) or (ii) with at least one and preferably more than one of the spacer probes as mentioned in table la or equivalents of thereof, under the appropiate hybridization and wash conditions, and/or with a taxon-specific probe derived from any of the spacer sequences as represented in figs. 1-103 under the same hybridization and wash conditions; (iv) detecting the hybrids formed in step (iii) with each of the probes used under appropiate hybridization and wash conditions; (v) identification of the micro-organism(s) present in the sample from the differential hybridization signals obtained in step (iv).

IPC 1-7

C12Q 1/68

IPC 8 full level

C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12R 1/01 (2006.01); C12R 1/32 (2006.01); C12R 1/35 (2006.01); C12R 1/38 (2006.01); C12R 1/42 (2006.01); C12R 1/44 (2006.01); C12R 1/46 (2006.01)

CPC (source: EP US)

C12Q 1/6888 (2013.01 - EP US); C12Q 1/689 (2013.01 - EP US); C12Q 2600/16 (2013.01 - EP US)

Citation (search report)

  • [A] WO 9304201 A1 19930304 - AMOCO CORP [US]
  • [A] EP 0452596 A1 19911023 - INNOGENETICS NV [BE] & WO 9116454 A1 19911031 - INNOGENETICS NV [BE]
  • [DA] EP 0395292 A2 19901031 - BARRY THOMAS GERARD [IE], et al
  • [X] TYLER S D ET AL: "Oligonucleotide primers designed to differentiate pathogenic pseudomonads on the basis of the sequencing of genes coding for 16S-23S rRNA internal transcribed spacer.", CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, vol. 2, no. 4, 1995, pages 448 - 453, XP000604143, ISSN: 1071-412X
  • [A] KOSTMAN J.R. ET AL.: "Molecular epidemiology of Pseudominas cepacia determined by polymerase chain reaction ribotyping", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 30, no. 8, August 1992 (1992-08-01), US, pages 2084 - 2087, XP000926225
  • [A] T.M. SCHMIDT, METHODS IN MOLECULAR AND CELLULAR BIOLOGY, vol. 5, no. 1, 1994, NEW YORK US, pages 3 - 12, XP002160264
  • [A] J.W. VAN DER GIESSEN ET AL., MICROBIOLOGY, vol. 140, no. 5, 1994, READING GB, pages 1103 - 1108, XP002160258
  • [A] Y. JI ET AL, MICROBIOLOGY, vol. 140, no. 1, 1994, READING GB, pages 123 - 132, XP002160259
  • [DA] R. FROTHINGHAM ET AL., JOURNAL OF BACTERIOLOGY, vol. 175, no. 10, 1993, BALTIMORE US, pages 2818 - 2825, XP002160260
  • [A] R. FROTHINGHAM ET AL., JOURNAL OF INFECTIOUS DISEASES, vol. 169, no. 2, 1994, CHICAGO US, pages 305 - 312, XP002160261
  • [A] Y. SUZUKI ET AL., JOURNAL OF BACTERIOLOGY, vol. 170, no. 6, 1988, BALTIMORE US, pages 2886 - 2889, XP002160262
  • [DA] K.E. KEMPSELL ET AL., JOURNAL OF GENERAL MICROBIOLOGY, vol. 138, no. 8, 1992, LONDON GB, pages 1717 - 1727, XP002160263
  • [XP] GILL S ET AL.: "Identification of variability of ribosomal DNA spacer from Pseudomonas soil isolates", CANADIAN JOURNAL OF MICROBIOLOGY, vol. 40, no. 7, July 1994 (1994-07-01), CA, pages 541 - 547, XP000926224

Designated contracting state (EPC)

AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 9600298 A1 19960104; AT E306560 T1 20051015; AT E423853 T1 20090315; AU 2924695 A 19960119; BR 9508101 A 19971230; CA 2193101 A1 19960104; CA 2193101 C 20100323; CZ 291483 B6 20030312; CZ 381996 A3 19980415; DE 69534516 D1 20051117; DE 69534516 T2 20060629; DE 69535923 D1 20090409; DK 0769068 T3 20060227; EP 0769068 A1 19970423; EP 0769068 B1 20051012; EP 1088899 A2 20010404; EP 1088899 A3 20010502; EP 1091004 A2 20010411; EP 1091004 A3 20010418; EP 1091004 B1 20090225; EP 1098006 A1 20010509; EP 1098007 A1 20010509; ES 2250971 T3 20060416; ES 2323161 T3 20090708; HK 1037687 A1 20030917; JP 3833703 B2 20061018; JP H10501976 A 19980224; RU 2154106 C2 20000810; US 2003215802 A1 20031120; US 2005142575 A1 20050630; US 6025132 A 20000215; US 6312903 B1 20011106; US 6811978 B2 20041102; US 7390623 B2 20080624

DOCDB simple family (application)

EP 9502452 W 19950623; AT 01200042 T 19950623; AT 95924923 T 19950623; AU 2924695 A 19950623; BR 9508101 A 19950623; CA 2193101 A 19950623; CZ 381996 A 19950623; DE 69534516 T 19950623; DE 69535923 T 19950623; DK 95924923 T 19950623; EP 01200037 A 19950623; EP 01200042 A 19950623; EP 01200045 A 19950623; EP 01200046 A 19950623; EP 95924923 A 19950623; ES 01200042 T 19950623; ES 95924923 T 19950623; HK 01107138 A 20011011; JP 50280496 A 19950623; RU 97100857 A 19950623; US 44889499 A 19991129; US 76533296 A 19961223; US 89511404 A 20040721; US 93148601 A 20010817