Global Patent Index - EP 1100885 A4

EP 1100885 A4 2001-12-12 - RAPID SUBCLONING USING SITE-SPECIFIC RECOMBINATION

Title (en)

RAPID SUBCLONING USING SITE-SPECIFIC RECOMBINATION

Title (de)

SCHNELLE SUBKLONIERUNG MITTELS ORTSSPEZIFISCHER REKOMBINATION

Title (fr)

SOUS-CLONAGE RAPIDE FAISANT APPEL A UNE RECOMBINAISON SPECIFIQUE A UN SITE

Publication

EP 1100885 A4 (EN)

Application

EP 98937141 A

Priority

  • US 9815481 W
  • US 12238498 A

Abstract (en)

[origin: WO0005355A1] The present invention provides compositions, including vectors, and methods for the rapid subcloning of nucleic acid sequences in vivo and in vitro. In particular, the invention provides vectors used to contain a gene of interest that comprise a sequence-specific recombinase target site. These vectors are used to rapidly transfer the gene or genes of interest into any vector that contains a sequence-specific recombinase target site located downstream of a regulatory element so that the gene of interest may be regulated.

IPC 1-7 (main, further and additional classification)

C12N 15/00; C12N 15/09; C12N 15/63; C12P 21/06

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 15/66 (2006.01); C12N 15/90 (2006.01); C40B 40/02 (2006.01); C40B 50/06 (2006.01)

CPC (invention and additional information)

C12N 15/905 (2013.01); C12N 15/66 (2013.01)

Citation (search report)

  • [LE] US 5851808 A 19981222 - ELLEDGE STEPHEN J [US], et al
  • [E] WO 9910488 A1 19990304 - SALK INST FOR BIOLOGICAL STUDI [US], et al
  • [E] WO 9921977 A1 19990506 - LIFE TECHNOLOGIES INC [US]
  • [Y] WO 9604393 A2 19960215 - DELTA & PINE LAND CO [US], et al
  • [A] EP 0220009 A2 19870429 - DU PONT [US]
  • [A] WO 9109957 A1 19910711 - DU PONT [US]
  • [A] WO 9315191 A1 19930805 - LIFE TECHNOLOGIES INC [US]
  • [X] SAUER B ET AL: "CONSTRUCTION OF ISOGENIC CELL LINES EXPRESSING HUMAN AND RAT ANGIOTENSIN II AT1 RECEPTORS BY CRE-MEDIATED SITE-SPECIFIC RECOMBINATION", METHODS: A COMPANION TO METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC., NEW YORK, NY, US, vol. 4, no. 2, 1992, pages 143 - 149, XP000613888, ISSN: 1046-2023
  • [X] AKAGI K ET AL: "CRE-MEDIATED SOMATIC SITE-SPECIFIC RECOMBINATION IN MICE", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 25, no. 9, 1997, pages 1766 - 1773, XP001004602, ISSN: 0305-1048
  • [X] QIN M ET AL: "CRE RECOMBINASE-MEDIATED SITE-SPECIFIC RECOMBINATION BETWEEN PLANT CHROMOSOMES", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 91, no. 5, 1 March 1994 (1994-03-01), pages 1706 - 1710, XP000615549, ISSN: 0027-8424
  • [X] ODELL J T ET AL: "SEED-SPECIFIC GENE ACTIVATION MEDIATED BY THE CRE/LOX SITE-SPECIFIC RECOMBINATION SYSTEM", PLANT PHYSIOLOGY, AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD, US, vol. 106, no. 2, 1 October 1994 (1994-10-01), pages 447 - 458, XP002912196, ISSN: 0032-0889
  • [T] LIU QINGHUA ET AL: "The univector plasmid-fusion system, a method for rapid construction of recombinant DNA without restriction enzymes.", CURRENT BIOLOGY, vol. 8, no. 24, 3 December 1998 (1998-12-03), pages 1300 - 1309, XP002155212, ISSN: 0960-9822
  • [Y] HALL RUTH M ET AL: "Mobile gene cassettes and integrons: Capture and spread of genes by site-specific recombination.", MOLECULAR MICROBIOLOGY, vol. 15, no. 4, 1995, pages 593 - 600, XP001028961, ISSN: 0950-382X
  • [Y] ELLEDGE S J ET AL: "LAMBDAYES A MULTIFUNCTIONAL COMPLEMENTARY DNA EXPRESSION VECTOR FOR THE ISOLATION OF GENES BY COMPLEMENTATION OF YEAST AND ESCHERICHIA-COLI MUTATIONS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 88, no. 5, 1991, 1991, pages 1731 - 1735, XP002916290, ISSN: 0027-8424
  • [Y] KILBY N J ET AL: "SITE-SPECIFIC RECOMBINASES: TOOLS FOR GENOME ENGINEERING", TRENDS IN GENETICS, ELSEVIER SCIENCE PUBLISHERS B.V. AMSTERDAM, NL, vol. 9, no. 12, 1993, pages 413 - 421, XP002916291, ISSN: 0168-9525
  • [Y] BOUHASSIRA ERIC E ET AL: "Transcriptional behavior of LCR enhancer elements integrated at the same chromosomal locus by recombinase-mediated cassette exchange.", BLOOD, vol. 90, no. 9, 1997, pages 3332 - 3344, XP002143981, ISSN: 0006-4971
  • [Y] SCHLAKE THOMAS ET AL: "Use of Mutated FLP Recognition Target (FRT) Sites for the Exchange of Expression Cassettes at Defined Chromosomal Loci.", BIOCHEMISTRY, vol. 33, no. 43, 1994, pages 12746 - 12751, XP000616165, ISSN: 0006-2960
  • [Y] ALADJEM MIRIT I ET AL: "Positive of FLP-mediated unequal sister chromatid exchange products in mammalian cells.", MOLECULAR AND CELLULAR BIOLOGY, vol. 17, no. 2, 1997, pages 857 - 861, XP002180200, ISSN: 0270-7306
  • [Y] ANGRAND PIERRE-OLIVIER ET AL: "Inducible expression based on regulated recombination: A single vector strategy for stable expression in cultured cells.", NUCLEIC ACIDS RESEARCH, vol. 26, no. 13, 1 July 1998 (1998-07-01), pages 3263 - 3269, XP001021788, ISSN: 0305-1048
  • See also references of WO 0005355A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 0005355 A1 20000203; AU 8592298 A 20000214; CA 2336520 A1 20000203; EP 1100885 A1 20010523; EP 1100885 A4 20011212; JP 2002522013 A 20020723

INPADOC legal status


2007-05-30 [18R] REFUSED

- Effective date: 20070413

2004-09-15 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20040730

2001-12-12 [A4] DESPATCH OF SUPPLEMENTARY SEARCH REPORT

- Effective date: 20011031

2001-12-12 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A4

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2001-08-01 [RIN1] INVENTOR CHANGED BEFORE GRANT

- Inventor name: ELLEDGE, STEPHEN, J.,DR.

2001-08-01 [RIN1] INVENTOR CHANGED BEFORE GRANT

- Inventor name: LIU, QINGHUA

2001-05-23 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20010214

2001-05-23 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE