Global Patent Index - EP 1100891 A2

EP 1100891 A2 2001-05-23 - EXPRESSION OF FUNCTIONAL EUKARYOTIC PROTEINS

Title (en)

EXPRESSION OF FUNCTIONAL EUKARYOTIC PROTEINS

Title (de)

DURCH DIREKTE EVOLUTION IN FUNKTIONELLER FORM EXPRIMIERBARE EUKARYONTISCHE PROTEINVARIANTEN IN E.COLI

Title (fr)

EXPRESSION DE PROTEINES EUCARYOTES FONCTIONNELLES

Publication

EP 1100891 A2 (EN)

Application

EP 99935983 A

Priority

  • US 9917127 W
  • US 9440398 P
  • US 24723299 A

Abstract (en)

[origin: WO0006718A2] This invention relates to the improved expression of evolved polynucleotide and polypeptide sequences encoding for eukaryotic enzymes, particularly peroxidase enzymes, in conventional or facile expression systems. Various methods for directed evolution of polynucleotide sequences can be used to obtain the improved sequences. The improved characteristics of the polypeptides or proteins generated in this manner include improved folding, without formation of inclusion bodies, and retained functional activity. In a particular embodiment, the invention relates to improved expression of the horseradish peroxidase gene and horseradish peroxidase enzymes.

IPC 1-7 (main, further and additional classification)

C12N 15/10; C12N 9/08; C12N 15/53; C12N 15/67; C12N 15/70; C12N 15/81

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 9/08 (2006.01); C12N 15/10 (2006.01); C12N 15/67 (2006.01); C12N 15/81 (2006.01); C12Q 1/26 (2006.01); C12Q 1/28 (2006.01); C12R 1/19 (2006.01); C12R 1/865 (2006.01)

CPC (invention and additional information)

C12N 15/1058 (2013.01); C12N 9/0065 (2013.01); C12N 15/102 (2013.01); C12N 15/67 (2013.01); C12N 15/815 (2013.01); C12Q 1/26 (2013.01); C12Q 1/28 (2013.01); C07K 2319/02 (2013.01); G01N 2333/795 (2013.01); G01N 2333/80 (2013.01); G01N 2333/90245 (2013.01)

Citation (search report)

See references of WO 0006718A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 0006718 A2 20000210; WO 0006718 A3 20000615; AU 5134599 A 20000221; CA 2331777 A1 20000210; EP 1100891 A2 20010523; IL 140509 D0 20020210; JP 2003503005 A 20030128; MX PA01000224 A 20021017

INPADOC legal status


2004-03-31 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20030917

2003-06-18 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20030506

2001-05-23 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20001229

2001-05-23 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2001-05-23 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO

- Free text: AL;LT;LV;MK;RO;SI