Global Patent Index - EP 1100957 A1

EP 1100957 A1 2001-05-23 - METHOD FOR IDENTIFICATION AND ISOLATION OF HEMATOPOIETIC PROGENITOR CELLS

Title (en)

METHOD FOR IDENTIFICATION AND ISOLATION OF HEMATOPOIETIC PROGENITOR CELLS

Title (de)

VERFAHREN ZUR IDENTIFIZIERUNG UND ISOLIERUNG VON HEMATOPOIETISCHEN VORLÄUFERZELLEN

Title (fr)

PROCEDE D'IDENTIFICATION ET D'ISOLATION DE CELLULES PROGENITRICES HEMATOPOIETIQUES

Publication

EP 1100957 A1 (EN)

Application

EP 99937573 A

Priority

  • US 9917096 W
  • US 12397098 A

Abstract (en)

[origin: WO0006766A1] There is provided a method for detecting and/or differentially identifying long-term repopulating hematopoietic progenitor cells. The method comprises detecting expression of tissue transglutaminase, which is uniquely expressed by long-term repopulating cells among blood or marrow cells. Thus, the method can comprise, for example, detecting the expressed protein, detecting an mRNA encoding the protein, or detecting an enzymatic metabolite of the protein. There is also provided a method for isolating long-term repopulating hematopoietic progenitor cells, comprising contacting cells from a sample of blood or marrow with an immobilized probe that selectively binds to tissue transglutaminase, to bind the long-term repopulating cells, and then collecting the bound cells. The selective identification or isolation of such long-term repopulating hematopoietic progenitor cells provides specific advantages, including the potentiation of allograft procedures, marrow reconstitution following myeloablation, and similar serious medical procedures.

IPC 1-7 (main, further and additional classification)

C12Q 1/48; C12Q 1/68; G01N 33/573

IPC 8 full level (invention and additional information)

C12Q 1/68 (2006.01); G01N 33/50 (2006.01); G01N 33/573 (2006.01)

CPC (invention and additional information)

C12Q 1/6876 (2013.01); G01N 33/5005 (2013.01); G01N 33/573 (2013.01); C12Q 2600/158 (2013.01)

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 0006766 A1 20000210; WO 0006766 A9 20000706; AU 5237899 A 20000221; CA 2335092 A1 20000210; EP 1100957 A1 20010523

INPADOC legal status


2002-09-18 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20020201

2001-07-11 [RIN1] INVENTOR (CORRECTION)

- Inventor name: VISSER, JAN W. M.

2001-07-11 [RIN1] INVENTOR (CORRECTION)

- Inventor name: IVANOVA, NATALIA B.

2001-05-23 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20010124

2001-05-23 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE