Global Patent Index - EP 1100966 A4

EP 1100966 A4 20060322 - IN SITU METHOD OF ANALYZING CELLS

Title (en)

IN SITU METHOD OF ANALYZING CELLS

Title (de)

IN SITU VERFAHREN ZUR GENANALYSE

Title (fr)

PROCEDE D'ANALYSE IN SITU DE CELLULES

Publication

EP 1100966 A4 20060322 (EN)

Application

EP 99935853 A 19990723

Priority

  • US 9916629 W 19990723
  • US 12270498 A 19980727

Abstract (en)

[origin: WO0006774A1] A method of in situ analysis of a biological sample comprising the steps of (a) staining the biological sample with N stains of which a first stain is selected from the group consisting of a first immunohistochemical stain, a first histological stain and a first DNA ploidy stain, and a second stain is selected from the group consisting of a second immunohistochemical stain, a second histological stain and a second DNA ploidy stain, with provisions that N is an integer greater than three and further that (i) if the first stain is the first immunohistochemical stain then the second stain is either the second histological stain or the second DNA ploidy stain; (ii) if the first stain is the first histological stain then the second stain is either the second immunohistochemical stain or the second DNA ploidy stain; whereas (iii) if the first stain is the first DNA ploidy stain then the second stain is either the second immunohistochemical stain or the second histological stain; and (b) using a spectral data collection device for collecting spectral data from the biological sample, the spectral data collection device and the N stains are selected such that a spectral component associated with each of the N stains is collectable.

IPC 1-7

C12Q 1/68

IPC 8 full level

G01N 33/53 (2006.01); C12Q 1/26 (2006.01); C12Q 1/28 (2006.01); C12Q 1/42 (2006.01); C12Q 1/68 (2006.01); G01J 3/28 (2006.01); G01J 3/457 (2006.01); G01N 21/78 (2006.01); G01N 33/50 (2006.01); G01N 33/533 (2006.01); G01N 33/569 (2006.01); G01N 33/58 (2006.01)

CPC (source: EP)

G01J 3/2823 (2013.01); G01J 3/457 (2013.01); G01N 33/5005 (2013.01); G01N 33/56966 (2013.01); G01J 2003/2866 (2013.01)

Citation (search report)

  • [X] US 4998284 A 19910305 - BACUS JAMES W [US], et al
  • [A] WO 9813525 A1 19980402 - APPLIED SPECTRAL IMAGING LTD [IL], et al
  • [X] LEVENSON R. M. AND FARKAS D. L.: "DIGITAL SPECTRAL IMAGING FOR HISTOPATHOLOGY AND CYTOPATHOLOGY", SPIE, vol. 2983, 1 May 1997 (1997-05-01), pages 123 - 135, XP008058932
  • [X] ROTHMANN C ET AL: "SPECTRAL IMAGING FOR QUANTITATIVE HISTOLOGY AND CYTOGENETICS", HISTOLOGY AND HISTOPATHOLOGY, MURCIA, ES, vol. 13, no. 3, 1 July 1998 (1998-07-01), pages 921 - 926, XP009048605, ISSN: 0213-3911
  • [X] BALABAN R S ET AL: "Microscopic spectral imaging using a video camera", JOURNAL OF MICROSCOPY 1986 UNITED KINGDOM, vol. 141, no. 1, 1986, pages 31 - 39, XP008058927
  • [A] ANDERSSON-ENGELS S ET AL INTERNATIONAL SOCIETY FOR OPTICAL ENGINEERING: "MULTICOLOR FLUORESCENCE IMAGING SYSTEM FOR TISSUE DIAGNOSTICS", BIOIMAGING AND TWO DIMENSIONAL SPECTROSCOPY. LOS ANGELES, JAN. 18 - 19, 1990, PROCEEDINGS OF SPIE, BELLINGHAM, SPIE, US, vol. VOL . 1205, 18 January 1990 (1990-01-18), pages 179 - 189, XP000218205
  • [A] MALIK Z ET AL: "FOURIER TRANSFORM MULTIPLEX SPECTROSCOPY FOR QUANTITATIVE CYTOLOGY", JOURNAL OF MICROSCOPY, OXFORD, GB, vol. 182, no. PART 2, May 1996 (1996-05-01), pages 133 - 140, XP009005240, ISSN: 0022-2720
  • [A] MALIK ZVI ET AL: "Subcellular localization of sulfonated tetraphenyl porphines in colon carcinoma cells by spectrally resolved imaging", PHOTOCHEMISTRY AND PHOTOBIOLOGY, vol. 65, no. 3, 1997, pages 389 - 396, XP008058934, ISSN: 0031-8655
  • [A] WOODBURN K W ET AL: "Localization and efficacy analysis of the phototherapeutic lutetium texaphyrin (PCI-0123) in the murine EMT6 sarcoma model", PHOTOCHEMISTRY AND PHOTOBIOLOGY, vol. 65, no. 3, 1997, pages 410 - 415, XP008058926, ISSN: 0031-8655
  • See references of WO 0006774A1

Designated contracting state (EPC)

DE FR GB IT NL SE

DOCDB simple family (publication)

WO 0006774 A1 20000210; AU 5123899 A 20000221; EP 1100966 A1 20010523; EP 1100966 A4 20060322; IL 140851 A0 20020210; JP 2002521682 A 20020716

DOCDB simple family (application)

US 9916629 W 19990723; AU 5123899 A 19990723; EP 99935853 A 19990723; IL 14085199 A 19990723; JP 2000562556 A 19990723