Global Patent Index - EP 1102791 A1

EP 1102791 A1 2001-05-30 - MODIFIED GREEN FLUORESCENT PROTEIN

Title (en)

MODIFIED GREEN FLUORESCENT PROTEIN

Title (de)

MODIFIZIERTES GRÜNES FLUORESZENZPROTEIN

Title (fr)

PROTEINE A FLUORESCENCE VERTE MODIFIEE

Publication

EP 1102791 A1 (EN)

Application

EP 99940293 A

Priority

  • GB 9902596 W
  • GB 9817225 A
  • GB 9817227 A
  • GB 9817229 A

Abstract (en)

[origin: WO0008444A1] A method for the measurement of a degree of fluorescence resonance energy transfer taking place between a donor and acceptor system by the steps of: irradiating a combined donor-acceptor system with a beam of intensity modulated excitation energy of a first wavelength; receiving fluorescence emissions from the donor and acceptor molecules having overlapping spectra; simultaneously determining a modulation lifetime ( tau mod) and a phase lifetime ( tau phi ) of the combined emitted fluorescence of the donor-acceptor system; and determining a degree of acceptor ingrowth by comparison of tau mod and tau phi . The method is particularly suited to measurements of a degree of FRET in a biological system.

IPC 1-7 (main, further and additional classification)

C07K 14/435; G01N 33/533

IPC 8 full level (invention and additional information)

C07K 14/435 (2006.01); C07K 19/00 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 15/09 (2006.01); C12Q 1/02 (2006.01); C12Q 1/48 (2006.01); G01N 21/64 (2006.01); G01N 21/78 (2006.01); G01N 33/50 (2006.01); G01N 33/68 (2006.01)

CPC (invention and additional information)

C07K 14/43595 (2013.01); G01N 21/6408 (2013.01)

Citation (search report)

See references of WO 0008054A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 0008444 A1 20000217; AU 5429199 A 20000228; AU 5429299 A 20000228; AU 5429399 A 20000228; EP 1102791 A1 20010530; EP 1102976 A1 20010530; EP 1102977 A1 20010530; JP 2002522040 A 20020723; JP 2002542453 A 20021210; WO 0008054 A1 20000217; WO 0008443 A1 20000217; WO 0008443 A9 20000525

INPADOC legal status


2003-12-17 [18W] WITHDRAWN

- Effective date: 20031021

2003-02-05 [RAP1] TRANSFER OF RIGHTS OF AN EP PUBLISHED APPLICATION

- Owner name: CANCER RESEARCH TECHNOLOGY LIMITED

2001-05-30 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20010305

2001-05-30 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2001-05-30 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO

- Free text: AL;LT;LV;MK;RO;SI