Global Patent Index - EP 1102976 A1

EP 1102976 A1 2001-05-30 - MULTIPLE FREQUENCY FLUORESCENCE LIFETIME IMAGING

Title (en)

MULTIPLE FREQUENCY FLUORESCENCE LIFETIME IMAGING

Title (de)

MEHRFREQUENZ-FLUORESZENZLEBENSDAUER-ABBILDUNGSVERFAHREN

Title (fr)

IMAGERIE DE DUREE DE VIE DE FLUORESCENCE A DES FREQUENCES MULTIPLES

Publication

EP 1102976 A1 (EN)

Application

EP 99940294 A

Priority

  • GB 9902597 W
  • GB 9817225 A
  • GB 9817227 A
  • GB 9817229 A

Abstract (en)

[origin: WO0008444A1] A method for the measurement of a degree of fluorescence resonance energy transfer taking place between a donor and acceptor system by the steps of: irradiating a combined donor-acceptor system with a beam of intensity modulated excitation energy of a first wavelength; receiving fluorescence emissions from the donor and acceptor molecules having overlapping spectra; simultaneously determining a modulation lifetime ( tau mod) and a phase lifetime ( tau phi ) of the combined emitted fluorescence of the donor-acceptor system; and determining a degree of acceptor ingrowth by comparison of tau mod and tau phi . The method is particularly suited to measurements of a degree of FRET in a biological system.

IPC 1-7 (main, further and additional classification)

G01N 21/64

IPC 8 full level (invention and additional information)

C07K 14/435 (2006.01); C07K 19/00 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 15/09 (2006.01); C12Q 1/02 (2006.01); C12Q 1/48 (2006.01); G01N 21/64 (2006.01); G01N 21/78 (2006.01); G01N 33/50 (2006.01); G01N 33/68 (2006.01)

CPC (invention and additional information)

C07K 14/43595 (2013.01); G01N 21/6408 (2013.01)

Citation (search report)

See references of WO 0008443A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 0008444 A1 20000217; AU 5429199 A 20000228; AU 5429299 A 20000228; AU 5429399 A 20000228; EP 1102791 A1 20010530; EP 1102976 A1 20010530; EP 1102977 A1 20010530; JP 2002522040 A 20020723; JP 2002542453 A 20021210; WO 0008054 A1 20000217; WO 0008443 A1 20000217; WO 0008443 A9 20000525

INPADOC legal status


2002-11-13 [18W] WITHDRAWN

- Date of withdrawal: 20020913

2001-05-30 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20010305

2001-05-30 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE