Global Patent Index - EP 1102983 A1

EP 1102983 A1 2001-05-30 - METHOD AND DEVICE FOR SEPARATING BIOMOLECULES

Title (en)

METHOD AND DEVICE FOR SEPARATING BIOMOLECULES

Title (de)

VERFAHREN UND VORRICHTUNG ZUR TRENNUNG VON BIOMOLEKÜLEN

Title (fr)

PROCEDE ET DISPOSITIF POUR SEPARER DES BIOMOLECULES

Publication

EP 1102983 A1 (DE)

Application

EP 99932728 A

Priority

  • DE 19831210 A
  • EP 9904411 W

Abstract (en)

[origin: DE19831210A1] The present invention relates to a method and to devices for the electrophoresis separation of gel biomolecules into one and two dimensions in an electrophoresis apparatus. This invention can essentially be used for separating proteins, glycoproteins, lipoproteins, nucleic acids or cellular complexes, etc. A first device according to this invention is characterised in that a combined electrophoresis chamber (1) comprises a central portion (2) with cooling members (3). These members are arranged under electrophoresis chambers (6, 7) which are formed on either side of the central portion (2) by a plurality of inner (4) and outer (5) plates interacting with isolation members (9) which can be removed or switched. The cooling members are also arranged under buffer vessels (8, 21). A second combined chamber is also provided for the electrophoresis separation of biomolecules or other mixtures of substances in the form of horizontally superposed gels into one or two dimensions, wherein said chamber comprises a base-wall plate as well as a cover plate. This invention further relates to a method for carrying out the separation into one and into two dimensions, as well as to the formulation of specific gels.

IPC 1-7 (main, further and additional classification)

G01N 27/447; B01D 57/02; C07K 1/26

IPC 8 full level (invention and additional information)

G01N 27/447 (2006.01)

CPC (invention and additional information)

G01N 27/44773 (2013.01)

Citation (search report)

See references of WO 0002039A1

Designated contracting state (EPC)

BE CH DE FR GB LI NL SE

EPO simple patent family

DE 19831210 A1 20000105; AU 4901099 A 20000124; CA 2336409 A1 20000113; EP 1102983 A1 20010530; WO 0002039 A1 20000113

INPADOC legal status


2006-06-21 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20060103

2004-08-04 [RAP1] TRANSFER OF RIGHTS OF AN EP PUBLISHED APPLICATION

- Owner name: WITA GMBH

2004-01-07 [RAP1] TRANSFER OF RIGHTS OF AN EP PUBLISHED APPLICATION

- Owner name: EUROGENTEC PROTEOMICS GMBH

2001-05-30 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20010323

2001-05-30 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): BE CH DE FR GB LI NL SE