EP 1104491 A4 20030129 - METHODS AND SYSTEMS FOR SEQUENCING DNA BY DISTINGUISHING THE DECAY TIMES OF FLUORESCENT PROBES
Title (en)
METHODS AND SYSTEMS FOR SEQUENCING DNA BY DISTINGUISHING THE DECAY TIMES OF FLUORESCENT PROBES
Title (de)
VERFAHREN UND SYSTEME ZUR SEQUENZIERUNG VON DNA MKITTELS UNTERSCHEIDUNG DER ZERFALLSZEITEN FLUORESZIERENDER SONDEN
Title (fr)
SEQUEN AGE D'ADN PAR DIFFERENCIATION DES PERIODES DE DECROISSANCE D'EMISSION DE SONDES FLUORESCENTES ET SYSTEMES A CET EFFET
Publication
Application
Priority
- US 9918294 W 19990811
- US 13255498 A 19980811
- US 12206498 P 19980811
- US 13218198 A 19980811
- US 21329798 A 19981215
Abstract (en)
[origin: WO0009753A1] Methods, apparatus and systems for distinguishing various fluorophores based on their fluorescence lifetimes. The techniques of the present invention use modulated radiation to irradiate fluorophores in a detection region. A fluorescence detector outputs a signal proportional to the detected fluorescence emissions, and a processor analyzes the proportional signal to determine fluorescence lifetimes. If the excitation source emits, or is modulated to emit, excitation pulses, the processor can measure the decay time directly; if the excitation source emits, or is modulated to emit, a sinusoidally varying excitation signal, the processor can determine the fluorescence lifetimes by measuring the phase difference or the demodulation relative to an excitation modulation reference signal. A method is provided for identifying components of a mixture by labeling the individual components with fluorescent agents having different fluorescence lifetimes. The components are subsequently separated, fluorescent labels detected and their lifetimes measured. Based on the measured fluorescent lifetimes, the components of mixtures of small organic molecules, polymers, peptides, saccharides and nucleic acids can be identified.
IPC 1-7
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: EP)
C12Q 1/6869 (2013.01)
C-Set (source: EP)
Citation (search report)
- [X] WO 9418218 A1 19940818 - SEQ LTD [US]
- [X] WO 9809154 A1 19980305 - BOEHRINGER MANNHEIM GMBH [DE], et al
- [A] EP 0753584 A1 19970115 - LI COR INC [US]
- [A] EP 0556509 A2 19930825 - HAMAMATSU PHOTONICS KK [JP]
- [A] WO 9000623 A1 19900125 - WALLAC OY [FI], et al
- [A] EP 0971038 A1 20000112 - LAB MOLECULAR BIOPHOTONICS [JP]
- [A] EP 0617286 A2 19940928 - WALLAC OY [FI]
- [A] WO 9627798 A1 19960912 - SOINI ERKKI [FI], et al
- [X] LI L-C ET AL: "On-the-fly fluorescence lifetime detection of labeled DNA primers", JOURNAL OF CHROMATOGRAPHY B: BIOMEDICAL SCIENCES & APPLICATIONS, ELSEVIER SCIENCE PUBLISHERS, NL, vol. 695, no. 1, 18 July 1997 (1997-07-18), pages 85 - 92, XP004125685, ISSN: 1570-0232
- See references of WO 0009753A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0009753 A1 20000224; AU 5479599 A 20000306; EP 1104491 A1 20010606; EP 1104491 A4 20030129
DOCDB simple family (application)
US 9918294 W 19990811; AU 5479599 A 19990811; EP 99941073 A 19990811