EP 1107990 A1 20010620 - HIGH-AFFINITY ANTIBODIES
Title (en)
HIGH-AFFINITY ANTIBODIES
Title (de)
ANTIKÖRPER MIT EINER HOHEN AFFINITÄT
Title (fr)
ANTICORPS A FORTE AFFINITE
Publication
Application
Priority
- GB 9902729 W 19990820
- GB 9818915 A 19980828
Abstract (en)
[origin: WO0012556A1] High-affinity monoclonal antibodies, wherein the affinity is characterised by: (i) incubating first and second samples of the antibody in antigen-coated microtitre plate wells at a concentration chosen to be within the linear part of a standard curve at pH 7.2 for 1 hour at 37 DEG C; (ii) removing unbound antibody from both samples; (iii) incubating the first sample with PBS at pH 7.2 for 1 hour at 37 DEG C, and reducing the pH of the second sample to pH 3 or below and incubating for 1 hour at 37 DEG C; (iv) removing unbound antibody from both samples; (v) incubating both samples with anti-antibody alkaline phosphatase-conjugate for 1 hour at 37 DEG C; (vi) removing unbound conjugate from both samples; and (vii) adding PNPP substrate to the samples, measuring the absorbance of the samples at 405 nm, and determining the amount of antibody bound to antigen, wherein the amount bound in the second sample is > 50 % of that of the first sample.
IPC 1-7
IPC 8 full level
A61K 39/395 (2006.01); A61K 47/48 (2006.01); A61P 35/00 (2006.01); C07K 1/22 (2006.01); G01N 33/577 (2006.01); C07K 16/30 (2006.01); C07K 16/32 (2006.01); C12N 15/09 (2006.01); C12N 15/13 (2006.01); C12P 21/08 (2006.01)
CPC (source: EP KR)
A61K 47/6815 (2017.08 - EP); A61P 35/00 (2018.01 - EP); C07K 16/30 (2013.01 - KR); C07K 16/3007 (2013.01 - EP); C07K 2317/20 (2013.01 - EP); C07K 2317/622 (2013.01 - EP)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0012556 A1 20000309; AU 5435099 A 20000321; BG 105294 A 20011229; BR 9913303 A 20011009; CA 2340865 A1 20000309; CN 1315966 A 20011003; EA 200100287 A1 20010827; EP 1107990 A1 20010620; GB 9818915 D0 19981021; HR P20010149 A2 20020228; HU P0103233 A2 20011228; HU P0103233 A3 20031028; ID 28905 A 20010712; IL 141523 A0 20020310; JP 2002525082 A 20020813; KR 20010089174 A 20010929; MX PA01002248 A 20020508; NO 20011006 D0 20010227; NO 20011006 L 20010227; PL 346472 A1 20020211; TR 200100643 T2 20010723; YU 15401 A 20030707; ZA 200101573 B 20020226
DOCDB simple family (application)
GB 9902729 W 19990820; AU 5435099 A 19990820; BG 10529401 A 20010226; BR 9913303 A 19990820; CA 2340865 A 19990820; CN 99810367 A 19990820; EA 200100287 A 19990820; EP 99940355 A 19990820; GB 9818915 A 19980828; HR P20010149 A 20010228; HU P0103233 A 19990820; ID 20010714 A 19990820; IL 14152399 A 19990820; JP 2000571062 A 19990820; KR 20017002434 A 20010226; MX PA01002248 A 19990820; NO 20011006 A 20010227; PL 34647299 A 19990820; TR 200100643 T 19990820; YU 15401 A 19990820; ZA 200101573 A 20010226