Title (en)

METHOD FOR PRODUCING HUMAN CLONED EMBRYOS BY EMPLOYING INTER-SPECIES NUCLEAR TRANSPLANTATION TECHNIQUE

Title (de)

VERFAHREN ZUR PRODUKTION VON GEKLONTEN, MENSCHLICHEN EMBRYOS MITTELS EINER INTERSPEZIES-KERNTRANSPLANTATIONSTECHNIK.

Title (fr)

PROCEDE D'OBTENTION D'EMBRYONS HUMAINS CLONES AU MOYEN DE TECHNIQUES DE TRANSPLANTATION INTER-ESPECES DE NOYAUX

Publication

EP 1109890 A4 20041229 (EN)

Application

EP 00941005 A 20000630

Priority

• KR 0000705 W 20000630
• KR 19990026163 A 19990630
• KR 19990026164 A 19990630
• KR 19990026165 A 19990630
• KR 19990026166 A 19990630
• KR 20000000206 A 20000104

Abstract (en)

[origin: WO0100793A1] The present invention provides a method for producing human cloned embryos by employing inter-species nuclear transplantation technique. The method for producing human cloned embryos of the invention comprises the steps of: preparing donor somatic cell lines collected from human; maturing oocytes collected from ovary of cow in vitro; removing the cumulus cells surrounding the oocytes; cutting a portion of zona pellucida of the matured oocytes to make a slit, and squeezing out a portion of cytoplasm including the first polar body through the slit to give enucleated recipient oocytes; transferring a nucleus to the recipient oocyte by injection of the donor cells to the enucleated recipient oocytes, followed by the subsequent electrofusion and activation of the electrofused cells to give embryos; and, postactivating and culturing the embryos in vitro. The human cloned embryos of the invention can be employed to obtain the human embryonic stem cells, which may be widely applied in biological and medical fields.

IPC 1-7

A01K 67/027; C12N 15/00

IPC 8 full level

A01K 67/027 (2006.01); C12N 5/06 (2006.01); C12N 5/07 (2010.01); C12N 5/071 (2010.01); C12N 5/08 (2006.01); C12N 5/10 (2006.01); C12N 15/00 (2006.01); C12N 15/09 (2006.01); C12N 15/87 (2006.01); C12R 1/91 (2006.01)

CPC (source: EP)

C12N 15/873 (2013.01)

Citation (search report)

• [X] WO 9807841 A1 19980226 - UNIV MASSACHUSETTS [US]
• [X] WO 9905266 A2 19990204 - WISCONSIN ALUMNI RES FOUND [US], et al
• [A] WO 9901163 A1 19990114 - UNIV MASSACHUSETTS [US]
• [X] ELIOT MARSHALL: "Claim of Human-Cow Embryo Greeted With Skepticism", SCIENCE, vol. 282, no. 5393, 1998, pages 1390 - 1391, XP002297337
• [A] MICHAEL BAKER: "HUMAN CLONING: Korean Report Sparks Anger and Inquiry", SCIENCE, vol. 283, no. 5398, 1 January 1999 (1999-01-01), pages 16 - 17, XP002297338
• [A] MICHAEL BAKER: "CLONING: Report Casts Doubt on Korean Experiment", SCIENCE, vol. 283, 29 January 1999 (1999-01-29), pages 617 - 619, XP002297339
• [AP] SOLTER D: "MAMMALIAN CLONING: ADVANCES AND LIMITATIONS", NATURE REVIEWS GENETICS, MACMILLAN MAGAZINES, GB, vol. 1, no. 3, December 2000 (2000-12-01), pages 199 - 207, XP001104859
• [T] WOO SUK HWANG ET AL.: "Evidence of a Pluripotent Human Embryonic Stem Cell Line Derived from a Cloned Blastocyst", SCIENCE, vol. 303, 12 March 2004 (2004-03-12), pages 1669 - 1674, XP002297340
• [T] G. VOGEL: "HUMAN CLONING:Scientists Take Step Toward Therapeutic Cloning", SCIENCE, vol. 303, no. 5660, 13 February 2004 (2004-02-13), pages 937 - 939, XP002297341
• [T] CIBELLI J B ET AL: "THE FIRST HUMAN CLONED EMBRYO", January 2002, SCIENTIFIC AMERICAN, SCIENTIFIC AMERICAN INC. NEW YORK, US, PAGE(S) 43-49, ISSN: 0036-8733, XP001126285
• See references of WO 0100793A1

Designated contracting state (EPC)

DE DK FR GB IT NL

DOCDB simple family (publication)

WO 0100793 A1 20010104; AU 5577600 A 20010131; CA 2334953 A1 20010104; CN 1304444 A 20010718; EP 1109890 A1 20010627; EP 1109890 A4 20041229; JP 2003503044 A 20030128; RU 2216591 C2 20031120

DOCDB simple family (application)

KR 0000705 W 20000630; AU 5577600 A 20000630; CA 2334953 A 20000630; CN 00800632 A 20000630; EP 00941005 A 20000630; JP 2001506787 A 20000630; RU 2000132213 A 20000630