Global Patent Index - EP 1111991 A4

EP 1111991 A4 20040310 - METHOD OF PERFORMING TRANSGENESIS

Title (en)

METHOD OF PERFORMING TRANSGENESIS

Title (de)

VERFAHREN ZUR DURCHFÜHRUNG VON TRANSGENESE

Title (fr)

PROCEDE DE MISE EN OEUVRE DE TRANSGENESE

Publication

EP 1111991 A4 20040310 (EN)

Application

EP 99942164 A 19990811

Priority

  • US 9918429 W 19990811
  • US 9607898 P 19980811
  • US 13425199 P 19990513

Abstract (en)

[origin: WO0008924A1] The invention provides a method for generating transgenic animals and cells by the coinsertion of nucleic acid and a nucleus into an unfertilized oocyte. Preferably, the coinsertion is by microinjection and more preferably by piezo-electrically actuated microinjection. Transgene (tg) expressing embryos are here produced following coinjection of unfertilized mouse oocytes with sperm heads and exogenous DNA encoding either a green fluorescent protein (GFP) or beta -galactosidase reporter. The microinjected oocyte may be allowed to develop into differentiated cells or stem cells; into an embryo in vitro prior to transfer into a host surrogate mother; or it may be transferred directly into a host surrogate mother. Embryonic development can occur to term, such that the offspring possess transgenic modifications that may alter their characteristics (phenotype) and are, in turn, transmitted to their offspring.

IPC 1-7

C12N 15/00; A01K 67/027; A01K 67/033; C12N 15/87; C12N 15/89

IPC 8 full level

A01K 67/027 (2006.01); A01K 67/033 (2006.01); C12N 5/10 (2006.01); C12N 15/09 (2006.01); C12N 15/87 (2006.01); C12N 15/89 (2006.01); C12R 1/91 (2006.01)

CPC (source: EP)

A01K 67/0275 (2013.01); A01K 67/0335 (2013.01); A01K 67/0338 (2013.01); C12N 15/87 (2013.01); C12N 15/89 (2013.01); A01K 2217/05 (2013.01)

Citation (search report)

  • [E] WO 9947642 A2 19990923 - UNIV HAWAII [US]
  • [Y] WAKAYAMA T ET AL: "Development of normal mice from oocytes injected with freeze-dried spermatozoa", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 16, 16 July 1998 (1998-07-16), pages 639 - 641, XP002117176, ISSN: 1087-0156
  • [XY] LAVITRANO M ET AL: "SPERM CELLS AS VECTORS FOR INTRODUCING FOREIGN DNA INTO EGGS: GENETIC TRANSFORMATION OF MICE", CELL, CELL PRESS, CAMBRIDGE, NA, US, vol. 57, no. 5, 2 June 1989 (1989-06-02), pages 717 - 723, XP000941006, ISSN: 0092-8674
  • [Y] KURETAKE S ET AL: "Fertilization and Development of mouse oocytes injected with isolated sperm heads", BIOLOGY OF REPRODUCTION, SOCIETY FOR THE STUDY OF REPRODUCTION, CHAMPAIGN, IL, US, vol. 55, no. 4, October 1996 (1996-10-01), pages 789 - 795, XP002117174, ISSN: 0006-3363
  • [Y] KIMURA Y ET AL: "ANALYSIS OF MOUSE OOCYTE ACTIVATION SUGGESTS THE INVOLVEMENT OF SPERM PERINUCLEAR MATERIAL", BIOLOGY OF REPRODUCTION, SOCIETY FOR THE STUDY OF REPRODUCTION, CHAMPAIGN, IL, US, vol. 58, no. 6, June 1998 (1998-06-01), pages 1407 - 1415, XP000938531, ISSN: 0006-3363
  • [Y] WAKAYAMA T ET AL: "Production of normal offspring from mouse oocytes injected with spermatozoa cryopreserved with or without cryoprotection.", JOURNAL OF REPRODUCTION AND FERTILITY. ENGLAND JAN 1998, vol. 112, no. 1, January 1998 (1998-01-01), pages 11 - 17, XP009004818, ISSN: 0022-4251
  • [XY] MAIONE B ET AL: "SPERM-MEDIATED GENE TRANSFER IN MICE", MOLECULAR REPRODUCTION AND DEVELOPMENT, LISSS, NEW YORK, NY, US, vol. 50, August 1998 (1998-08-01), pages 406 - 409, XP000941059, ISSN: 1040-452X
  • [A] REVENTOS J ET AL: "TRANSGENIC ANIMAL MODELS IN REPRODUCTIVE ENDOCRINE RESEARCH", EUROPEAN JOURNAL OF ENDOCRINOLOGY, SCANDINAVIAN UNIVERSITY PRESS, NO, vol. 136, no. 6, 1997, pages 566 - 580, XP001011890, ISSN: 0804-4643
  • [PX] PERRY A C F ET AL: "MAMMALIAN TRANSGENESIS BY INTRACYTOPLASMIC SPERM INJECTION", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 284, 14 May 1999 (1999-05-14), pages 1180 - 1183, XP000828092, ISSN: 0036-8075
  • See references of WO 0008924A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 0008924 A1 20000224; WO 0008924 A9 20000525; BR 9913644 A 20011120; CA 2340242 A1 20000224; CN 1316878 A 20011010; EP 1111991 A1 20010704; EP 1111991 A4 20040310; IL 141362 A0 20020310; JP 2002524054 A 20020806; MX PA01001586 A 20020408; NZ 509861 A 20040130

DOCDB simple family (application)

US 9918429 W 19990811; BR 9913644 A 19990811; CA 2340242 A 19990811; CN 99810645 A 19990811; EP 99942164 A 19990811; IL 14136299 A 19990811; JP 2000564438 A 19990811; MX PA01001586 A 19990811; NZ 50986199 A 19990811