EP 1114173 A4 20050216 - SYNTHESIS OF VANILLIN FROM A CARBON SOURCE
Title (en)
SYNTHESIS OF VANILLIN FROM A CARBON SOURCE
Title (de)
SYNTHESE VON VANILLIN AUS EINER KOHLENSTOFFQUELLE
Title (fr)
SYNTHESE DE LA VANILLINE A PARTIR D'UNE SOURCE DE CARBONE
Publication
Application
Priority
- US 9921323 W 19990916
- US 10093798 P 19980918
Abstract (en)
[origin: WO0017319A2] A bioengineered synthesis scheme for the production of vanillin from a carbon source is provided. The bioconversion methods of the present invention comprise the steps of microbe-catalyzed conversion of a carbon source to vanillic acid followed by enzyme-catalyzed reduction of the vanillic acid to produce vanillin. As shown in the synthesis scheme of Figure 2, the microbe-catalyzed conversion step of the present invention requires five enzymes which are provided by a recombinant microbe. In a preferred embodiment, the recombinant microbe is Escherichia coli designed to cause dehydration of 3-dehydroshikimic acid and regioselective methylation of the resulting protocatechuic acid. The enzyme-catalyzed reduction step of the present invention comprises the reduction of vanillic acid to vanillin by aryl-aldehyde dehydrogenase.
IPC 1-7
IPC 8 full level
C12N 9/02 (2006.01); C12N 15/52 (2006.01); C12P 7/24 (2006.01)
CPC (source: EP KR US)
C12N 9/0008 (2013.01 - EP US); C12N 15/52 (2013.01 - EP US); C12P 7/24 (2013.01 - EP KR US)
Citation (search report)
- [A] US 5776736 A 19980707 - FROST JOHN W [US], et al
- [Y] US 5262315 A 19931116 - GROSS BRIGITTE [FR], et al
- [YA] EP 0857789 A2 19980812 - KYOWA HAKKO KOGYO KK [JP]
- [YA] US 5795759 A 19980818 - ROSAZZA JOHN P N [US], et al
- [YA] BARGHINI P ET AL: "Optimal conditions for bioconversion of ferulic acid into vanillic acid by Pseudomonas fluorescens BF13 cells", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 49, no. 3, March 1998 (1998-03-01), pages 309 - 314, XP002311447, ISSN: 0175-7598
- [XA] LESAGE-MEESSEN LAURENCE ET AL: "A two-step bioconversion process for vanillin production from ferulic acid combining Aspergillus niger and Pycnoporus cinnabarinus", JOURNAL OF BIOTECHNOLOGY, vol. 50, no. 2-3, 1996, pages 107 - 113, XP002311448, ISSN: 0168-1656
- [YA] LESAGE-MEESSEN L ET AL: "An attempt to channel the transformation of vanillic acid into vanillin by controlling methoxyhydroquinone formation in Pycnoporus cinnabarinus with cellobiose", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 47, no. 4, 1997, pages 393 - 397, XP002311449, ISSN: 0175-7598
- [YA] THIBAULT JEAN-FRANCOIS ET AL: "Fungal bioconversion of agricultural by-products to vanillin", LEBENSMITTEL-WISSENSCHAFT AND TECHNOLOGIE, vol. 31, no. 6, 1998, pages 530 - 536, XP002311450, ISSN: 0023-6438
- [A] SNELL K D ET AL: "Synthetic modification of the Escherichia coli chromosome: Enhancing the biocatalytic conversion of glucose into aromatic chemicals", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 1996 UNITED STATES, vol. 118, no. 24, 1996, pages 5605 - 5614, XP002311451, ISSN: 0002-7863
- [A] KRINGS U ET AL: "Biotechnological production of flavours and fragrances", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 49, no. 1, January 1998 (1998-01-01), pages 1 - 8, XP002311452, ISSN: 0175-7598
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0017319 A2 20000330; WO 0017319 A3 20000727; AU 6043399 A 20000410; CA 2344665 A1 20000330; EP 1114173 A2 20010711; EP 1114173 A4 20050216; KR 20010085827 A 20010907; US 6372461 B1 20020416
DOCDB simple family (application)
US 9921323 W 19990916; AU 6043399 A 19990916; CA 2344665 A 19990916; EP 99969432 A 19990916; KR 20017003704 A 20010319; US 39772099 A 19990916