EP 1117776 A1 20010725 - ENZYMATIC SYNTHESIS OF ssDNA
Title (en)
ENZYMATIC SYNTHESIS OF ssDNA
Title (de)
ENZYMATISCHE SYNTHESE VON SSDNA
Title (fr)
SYNTHESE ENZYMATIQUE D'ADN SIMPLE BRIN
Publication
Application
Priority
- US 9923933 W 19991012
- US 16979398 A 19981009
- US 39778399 A 19990916
Abstract (en)
[origin: WO0022113A1] Methods and compositions for producing single-stranded cDNA (ss-cDNA) with a vector-based system in eukaryotic cells. The vector contains all necessary signaling instructions and enzymatic functions to allow the host cell to produce the ssDNA encoding a desired nucleic acid sequence (a "sequence of interest"). Described are the components included in the vector for synthesizing ssDNA in vivo. They include (1) a reverse transcriptase gene, (2) a genetic element that supplies the template for the desired ssDNA sequence of interest, and (3) a second genetic element located proximal to the genetic element encoding the sequence of interest that supplies the primer site for reverse transcription by the reverse transcriptase molecule. The vector also contains appropriate promoter(s)/enhancer(s). Also described herein is a method to construct a vector including these components.
IPC 1-7
C12N 15/10; C12N 15/11; C12N 15/85; C12N 5/10; A61K 31/711; A61K 48/00
IPC 8 full level
A61K 31/711 (2006.01); A61K 35/74 (2006.01); A61K 35/76 (2006.01); A61K 48/00 (2006.01); A61P 9/00 (2006.01); A61P 11/06 (2006.01); A61P 13/12 (2006.01); A61P 25/00 (2006.01); A61P 25/14 (2006.01); A61P 25/16 (2006.01); A61P 29/00 (2006.01); A61P 31/12 (2006.01); A61P 31/18 (2006.01); A61P 31/22 (2006.01); A61P 35/00 (2006.01); A61P 37/06 (2006.01); A61P 43/00 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 5/10 (2006.01); C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12N 15/11 (2006.01); C12N 15/113 (2010.01); G01N 33/15 (2006.01); G01N 33/50 (2006.01)
CPC (source: EP KR)
A61P 9/00 (2017.12 - EP); A61P 11/06 (2017.12 - EP); A61P 13/12 (2017.12 - EP); A61P 25/00 (2017.12 - EP); A61P 25/14 (2017.12 - EP); A61P 25/16 (2017.12 - EP); A61P 29/00 (2017.12 - EP); A61P 31/12 (2017.12 - EP); A61P 31/18 (2017.12 - EP); A61P 31/22 (2017.12 - EP); A61P 35/00 (2017.12 - EP); A61P 37/06 (2017.12 - EP); A61P 43/00 (2017.12 - EP); C12N 15/10 (2013.01 - EP); C12N 15/113 (2013.01 - EP); C12N 15/63 (2013.01 - KR); C12N 2310/111 (2013.01 - EP); C12N 2310/12 (2013.01 - EP); C12N 2310/15 (2013.01 - EP); C12N 2310/53 (2013.01 - EP)
Citation (search report)
See references of WO 0022113A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0022113 A1 20000420; WO 0022113 A9 20000824; AU 6430599 A 20000501; BR 9914772 A 20011211; CA 2346155 A1 20000420; EP 1117776 A1 20010725; IL 142490 A0 20020310; JP 2002527061 A 20020827; KR 20010099682 A 20011109; MX PA01003642 A 20030721
DOCDB simple family (application)
US 9923933 W 19991012; AU 6430599 A 19991012; BR 9914772 A 19991012; CA 2346155 A 19991012; EP 99951989 A 19991012; IL 14249099 A 19991012; JP 2000576004 A 19991012; KR 20017004474 A 20010409; MX PA01003642 A 19991012