EP 1121458 A1 20010808 - METHODS FOR ASSESSING COMPLEMENT ACTIVATION
Title (en)
METHODS FOR ASSESSING COMPLEMENT ACTIVATION
Title (de)
VERFAHREN ZUR BESTIMMUNG DER KOMPLEMENTAKTIVIERUNG
Title (fr)
METHODE D'EVALUATION DE L'ACTIVATION DU COMPLEMENT
Publication
Application
Priority
- US 9924150 W 19991015
- US 17357998 A 19981015
- US 24582999 A 19990205
Abstract (en)
[origin: WO0022160A1] Methods for measuring in vivo activation of the lectin pathway by measuring mannan-binding serine protease activity (MASP) are provided. The methods are accomplished by measuring C3a and C4a levels in in vitro activated EDTA plasma. In particular, the increase in C3a and/or C4a as a function of time is an indicator of the amount of activated MASP in EDTA plasma. Methods are also provided for measuring the alternate and classical pathways of complement activation, exclusive of the lectin pathway, and thereby disorders associated therewith. To perform such measurements, Futhan or other serine protease inhibitor is added to blood or plasma, containing a divalent metal ion chelator, and C3a and C4a are measured.
IPC 1-7
IPC 8 full level
C12Q 1/37 (2006.01)
CPC (source: EP US)
C12Q 1/37 (2013.01 - EP US)
Citation (search report)
See references of WO 0022160A1
Designated contracting state (EPC)
DE ES FR GB IT
DOCDB simple family (publication)
WO 0022160 A1 20000420; WO 0022160 A9 20000824; AU 1117100 A 20000501; EP 1121458 A1 20010808; US 6297024 B1 20011002
DOCDB simple family (application)
US 9924150 W 19991015; AU 1117100 A 19991015; EP 99954951 A 19991015; US 24582999 A 19990205