EP 1121467 A2 20010808 - AUTOMATABLE QUICK TEST FOR DIRECT DETECTION OF APC RESISTANCE MUTATION WITH SPECIFIC PRIMERS AND ASSAY
Title (en)
AUTOMATABLE QUICK TEST FOR DIRECT DETECTION OF APC RESISTANCE MUTATION WITH SPECIFIC PRIMERS AND ASSAY
Title (de)
EIN AUTOMATISIERBARER SCHNELLTEST ZUM DIREKTEN NACHWEIS DER APC RESISTENZ MUTATION MIT SPEZIFISCHEN PRIMERN UND OLIGONUKLEOTIDEN ZUR DETEKTION
Title (fr)
TEST RAPIDE POUVANT ETRE AUTOMATISE POUR LA DETECTION DIRECTE DE LA MUTATION ASSOCIEE A LA RESISTANCE INDUITE PAR LA PROTEINE C ACTIVEE (APC) AU MOYEN D'AMORCES ET DE SONDES SPECIFIQUES
Publication
Application
Priority
- DE 19848665 A 19981022
- EP 9907609 W 19991011
Abstract (en)
[origin: DE19848665A1] Primers (I) derived from the factor V gene are new. (I) have formulae (1), specific for the G to A mutation at position 1691 in exon 10; (2), specific for the wild-type sequence and (3), a reverse primer for use with both (1) and (2): (1) ACA4TACCTGTATTCCAT; (2) ACA4TACCTGTATTCCGC; (3) TCATGAAATAACTTTGCAAATGA . Independent claims are also included for the following: (1) oligonucleotide probes (II) of formulae (4)-(7): (a) TACTTATAAGTGGAACATCTTAGAGTTTGATGAACC; (b) TGCCCAGTGCTTAACAAGACCATACTACAGTGACGT ; (c) UACUUAUAAGUGGAACAUCUUAGAGUUUGAUGAACC; (d) UACUUAUAAGUGGAACAUCUUAGAGUUUGA; (2) sets of primers comprising (3) plus (1) or (2); (3) a method for detecting factor V gene sequences by amplifying sample DNA with both primer sets of (b) and evaluating amplification; and (4) a test kit for detecting APC (activated protein C)-resistance mutations comprising one or more (I) and optionally one or more (II).
IPC 1-7
IPC 8 full level
C12Q 1/68 (2006.01); C12Q 1/6883 (2018.01)
CPC (source: EP)
C12Q 1/6883 (2013.01); C12Q 2600/156 (2013.01)
Citation (search report)
See references of WO 0024928A2
Designated contracting state (EPC)
CH DE FR GB LI NL SE
DOCDB simple family (publication)
DE 19848665 A1 20000427; AU 6337699 A 20000515; EP 1121467 A2 20010808; WO 0024928 A2 20000504; WO 0024928 A3 20000622
DOCDB simple family (application)
DE 19848665 A 19981022; AU 6337699 A 19991011; EP 9907609 W 19991011; EP 99950696 A 19991011