EP 1127114 A1 20010829 - METHOD OF ISOLATING PLASMID DNA
Title (en)
METHOD OF ISOLATING PLASMID DNA
Title (de)
VERFAHREN ZUR ISOLIERUNG VON PLASMID-DNA
Title (fr)
PROCEDE POUR L'ISOLEMENT DE PLASMIDE D'ADN
Publication
Application
Priority
- DE 19851156 A 19981106
- EP 9908091 W 19991026
Abstract (en)
[origin: DE19851156C1] Isolating plasmid DNA from a microbial culture, is new. Isolating plasmid DNA from a microbial culture, is new and comprises: (1) acidifying the culture, mixing it with particles, incubating the mixture to immobilize the microbial cells on the particles, and separating the particles; (2) resuspending the cell-bearing particles, lyzing the cells, adding a neutralizing buffer, incubating the mixture, and separating and discarding the particles; and (3) adding particles to the supernatant, incubating the mixture, separating the supernatant, and eluting plasmid DNA from the particles with an elution buffer.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12N 1/00 (2006.01); C12N 15/10 (2006.01); C12R 1/19 (2006.01)
CPC (source: EP US)
C12N 15/1006 (2013.01 - EP US); C12N 15/1013 (2013.01 - EP US)
Citation (search report)
See references of WO 0029562A1
Designated contracting state (EPC)
DE FR GB
DOCDB simple family (publication)
DE 19851156 C1 20000427; EP 1127114 A1 20010829; JP 2003521228 A 20030715; US 6548253 B1 20030415; WO 0029562 A1 20000525
DOCDB simple family (application)
DE 19851156 A 19981106; EP 9908091 W 19991026; EP 99969895 A 19991026; JP 2000582546 A 19991026; US 83095601 A 20010608