Title (en)

TRANSGENIC ANIMALS

Title (de)

TRANSGENE TIERE

Title (fr)

ANIMAUX TRANSGENIQUES

Publication

EP 1130960 A4 20030730 (EN)

Application

EP 99960310 A 19991112

Priority

• US 9926848 W 19991117
• US 19667098 A 19981119

Abstract (en)

[origin: WO0030437A1] The present invention provides improved methods and compositions for the generation of transgenic non-human animals. The present invention permits the introduction of exogenous 5nucleic acid sequences into the genome of unfertilized eggs (e.g., pre-maturation oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus into the perivitelline space of the egg. The methods of the present invention provide an increased efficiency of production of transgenic animals with a reduced rate of generating animals which are mosaic for the presence of the transgene.

IPC 1-7

A01K 67/027; C12P 21/00; C12N 15/00

IPC 8 full level

A01K 67/027 (2006.01); C07K 14/02 (2006.01); C12N 15/85 (2006.01)

CPC (source: EP)

A01K 67/0275 (2013.01); C07K 14/005 (2013.01); C12N 15/8509 (2013.01); A01K 2217/05 (2013.01); A01K 2227/101 (2013.01); A01K 2267/01 (2013.01); C12N 2730/10122 (2013.01)

Citation (search report)

• [PX] CHAN A W S ET AL: "Transgenic cattle produced by reverse-transcribed gene transfer in oocyte", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 95, no. 24, 24 November 1998 (1998-11-24), pages 14028 - 14033, XP002173620, ISSN: 0027-8424
• [A] BURNS JANE C ET AL: "Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: Concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 90, no. 17, 1993, 1993, pages 8033 - 8037, XP002231450, ISSN: 0027-8424
• [DA] HASKELL R E ET AL: "EFFICIENT PRODUCTION OF TRANSGENIC CATTLE BY RETROVIRAL INFECTION OF EARLY EMBRYOS", MOLECULAR REPRODUCTION AND DEVELOPMENT, LISSS, NEW YORK, NY, US, vol. 40, no. 3, March 1995 (1995-03-01), pages 386 - 390, XP001002645, ISSN: 1040-452X
• See references of WO 0030437A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 0030437 A1 20000602; WO 0030437 A8 20010419; WO 0030437 A9 20010517; AU 1721000 A 20000613; AU 772439 B2 20040429; CA 2351553 A1 20000602; CA 2351553 C 20070130; EP 1130960 A1 20010912; EP 1130960 A4 20030730; NZ 511808 A 20040130

DOCDB simple family (application)

US 9926848 W 19991117; AU 1721000 A 19991117; CA 2351553 A 19991112; EP 99960310 A 19991112; NZ 51180899 A 19991117