EP 1133513 A1 20010919 - MULTIDOMAIN POLYNUCLEOTIDE MOLECULAR SENSORS
Title (en)
MULTIDOMAIN POLYNUCLEOTIDE MOLECULAR SENSORS
Title (de)
AUS MEHREREN DOMÄNEN VON POLYNUKLEOTIDEN BESTEHENDE MOLEKULARE SENSOREN
Title (fr)
DETECTEURS MOLECULAIRES POLYNUCLEOTIDIQUES A DOMAINES MULTIPLES
Publication
Application
Priority
- US 9925497 W 19991029
- US 10682998 P 19981103
- US 12668399 P 19990329
Abstract (en)
[origin: WO0026226A1] Multidomain polynucleotides responsive to signalling agents are designed and constructed to have at least three domains which can be partially or completely overlapping or nonoverlapping: an actuator (catalytic or reporter) domain, a bridging domain, and a receptor domain. In a typical embodiment, a signalling agent such as a chemical ligand interacts with the receptor domain, which changes conformation or otherwise influences the bridging domain so that the activity, catalytic, or reporter function of the actuator domain is stimulated or inhibited. In some ribozyme embodiments, for example, ligand-specific molecular sensors composed of RNA are created by coupling pre-existing catalytic and receptor domains via novel structural bridges which function such that binding of a ligand to the receptor domain triggers a conformational change within the bridge, and this structural reorganization dictates the activity of the adjoining ribozyme. Processes for allosterically selecting other multidomain polynucleotides typically involve mixing and matching domains to optimize binding or other signal response and/or reporter activity.
IPC 1-7
IPC 8 full level
G01N 33/50 (2006.01); C07H 21/00 (2006.01); C12N 9/00 (2006.01); C12N 11/00 (2006.01); C12N 15/09 (2006.01); C12N 15/113 (2010.01); C12Q 1/68 (2006.01); G01N 33/566 (2006.01)
CPC (source: EP)
C07H 21/00 (2013.01); C12N 15/1048 (2013.01); C12N 15/113 (2013.01); C07K 2319/00 (2013.01); C12N 2310/121 (2013.01); C12N 2310/18 (2013.01)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0026226 A1 20000511; AU 1601300 A 20000522; AU 2004203816 A1 20040902; AU 772881 B2 20040513; CA 2348779 A1 20000511; EP 1133513 A1 20010919; EP 1133513 A4 20020703; JP 2002528109 A 20020903
DOCDB simple family (application)
US 9925497 W 19991029; AU 1601300 A 19991029; AU 2004203816 A 20040811; CA 2348779 A 19991029; EP 99958709 A 19991029; JP 2000579614 A 19991029