EP 1133514 A1 20010919 - METHOD FOR THE ELECTROCHEMICAL DETECTION OF NUCLEIC ACID OLIGOMER HYBRIDS
Title (en)
METHOD FOR THE ELECTROCHEMICAL DETECTION OF NUCLEIC ACID OLIGOMER HYBRIDS
Title (de)
VERFAHREN ZUR ELEKTROCHEMISCHEN DETEKTION VON NUKLEINSÄUREOLIGOMERHYBRIDEN
Title (fr)
PROCEDE DE DETECTION ELECTROCHIMIQUE D'HYBRIDES D'ACIDES NUCLEIQUES-OLIGOMERES
Publication
Application
Priority
- DE 19853957 A 19981123
- DE 19921940 A 19990429
- EP 9908888 W 19991119
Abstract (en)
[origin: US7056664B1] The invention relates to a method for the electrochemical detection of sequence-specific nucleic acid oligomer hybridization events. To this end single DNA/RNA/PNA oligomer strands which at one end are covalently joined to a support surface and at the other, free end, covalently linked to a redox pair, are used as hybridization matrix (probe). As a result of treatment with the oligonucleotide solution (target) to be examined, the electric communication between the conductive support surface and the redox pair bridged by the single-strand oligonucleotide, which communication initially is either absent or very weak, is modified. In case of hybridization, the electric communication between the support surface and the redox pair, which is now bridged by a hybridized double-strand oligonucleotide, is increased. This permits the detection of a hybridization event by electrochemical methods such as cyclic voltametry, amperometry or conductivity measurement.
IPC 1-7
IPC 8 full level
C07H 21/00 (2006.01); C07H 21/02 (2006.01); C07H 21/04 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); G01N 27/12 (2006.01); G01N 27/30 (2006.01); G01N 27/416 (2006.01); G01N 27/48 (2006.01); G01N 33/483 (2006.01); G01N 33/50 (2006.01); G01N 33/566 (2006.01)
CPC (source: EP US)
C07H 21/00 (2013.01 - EP US); C12Q 1/6825 (2013.01 - EP US); C12Q 1/6874 (2013.01 - EP)
Citation (search report)
See references of WO 0031101A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
US 7056664 B1 20060606; AU 1383600 A 20000613; AU 751220 B2 20020808; BR 9915526 A 20011113; CA 2345157 A1 20000602; CN 1324365 A 20011128; DE 19964220 C2 20030703; EP 1133514 A1 20010919; IL 142758 A0 20020310; JP 2002532386 A 20021002; PL 348144 A1 20020506; RU 2213095 C2 20030927; TR 200101179 T2 20011121; WO 0031101 A1 20000602
DOCDB simple family (application)
US 85654301 A 20011213; AU 1383600 A 19991119; BR 9915526 A 19991119; CA 2345157 A 19991119; CN 99812448 A 19991119; DE 19964220 A 19990429; EP 9908888 W 19991119; EP 99972637 A 19991119; IL 14275899 A 19991119; JP 2000583928 A 19991119; PL 34814499 A 19991119; RU 2001114192 A 19991119; TR 200101179 T 19991119