EP 1135526 A2 20010926 - METHOD FOR IDENTIFYING CYTOSINE METHYLATION PATTERNS IN GENOMIC DNA
Title (en)
METHOD FOR IDENTIFYING CYTOSINE METHYLATION PATTERNS IN GENOMIC DNA
Title (de)
VERFAHREN ZUR IDENTIFIKATION VON CYTOSIN-METHYLIERUNGSMUSTERN IN GENOMISCHER DNA
Title (fr)
PROCEDE D'IDENTIFICATION DE MODELES DE METHYLATION DE CYTOSINE DANS L'ADN GENOMIQUE
Publication
Application
Priority
- DE 9903747 W 19991119
- DE 19853398 A 19981119
Abstract (en)
[origin: WO0031294A2] The invention relates to a method for identifying 5-methylcytosine positions in genomic DNA. The method is characterised by the following steps: a) the genomic DNA of a cell, a cell line, a tissue or an individual is chemically treated in such a way that the cytosine and 5-methylcytosine react differently and the two products present different base pairing behaviour in the duplex; b) the same nucleic acid section is amplified by means of a polymerase reaction; c) the same nucleic acid section of at least one other cell, cell line, tissue or individual or of any given reference DNA is treated according to steps a) and b); d) heteroduplexes are formed from the at least two amplification products obtained in steps b) and c); and e) a detectable marking is introduced into the heteroduplexes by means of a reaction that is specific to non-complementary base pairs.
IPC 1-7
IPC 8 full level
G01N 27/62 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6809 (2018.01); C12Q 1/6858 (2018.01); G01N 33/50 (2006.01)
CPC (source: EP)
C12Q 1/6809 (2013.01); C12Q 1/6858 (2013.01)
Citation (search report)
See references of WO 0031294A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0031294 A2 20000602; WO 0031294 A3 20001019; AU 1856500 A 20000613; AU 757473 B2 20030220; CA 2351143 A1 20000602; DE 19853398 C1 20000316; EP 1135526 A2 20010926; IL 143176 A0 20020421; IL 143176 A 20050517; IS 5916 A 20010410; JP 2002530118 A 20020917; NZ 511638 A 20031128
DOCDB simple family (application)
DE 9903747 W 19991119; AU 1856500 A 19991119; CA 2351143 A 19991119; DE 19853398 A 19981119; EP 99962069 A 19991119; IL 14317699 A 19991119; IS 5916 A 20010410; JP 2000584101 A 19991119; NZ 51163899 A 19991119