Global Patent Index - EP 1137784 A1

EP 1137784 A1 20011004 - METHOD FOR ISOLATING AND SELECTING GENES CODING FOR ENZYMES, AND SUITABLE CULTURE MEDIUM

Title (en)

METHOD FOR ISOLATING AND SELECTING GENES CODING FOR ENZYMES, AND SUITABLE CULTURE MEDIUM

Title (de)

VERFAHREN ZUR ISOLIERUNG UND SELEKTION VON FÜR ENZYME-KODIERENDE GENE, UND DAFÜR GEEIGNETES KULTURMEDIUM

Title (fr)

METHODE D'ISOLEMENT ET DE SELECTION DE GENES CODANT POUR DES ENZYMES, ET MILIEU DE CULTURE APPROPRIE

Publication

EP 1137784 A1 20011004 (FR)

Application

EP 99958292 A 19991210

Priority

  • FR 9903089 W 19991210
  • FR 9815849 A 19981211
  • FR 9909489 A 19990719

Abstract (en)

[origin: FR2787121A1] The invention concerns a method for selecting and/or isolating DNA sequences coding for enzymes involved in the biological conversion of an appropriate substrate consisting of methionine and its derivatives, such as HMTBS, said method comprising the following steps: 1) cloning DNA sequences in a carrier enabling their expression in an appropriate host micro-organism; 2) transforming a suitable auxotroph micro-organisms for methionine by introducing carriers previously obtained in said suitable micro-organism; 3) growing the transformed micro-organisms previously obtained in a suitable culture medium comprising a sufficient amount of appropriate substrate; and 4) selecting the transformed micro-organisms capable of growing in the appropriate culture medium; and 5) isolating and as the case may be identifying the DNA sequences involved in the biological conversion of the appropriate substrate.

IPC 1-7

C12N 15/55; C12N 9/78; C12P 13/12; C12N 1/21

IPC 8 full level

C12N 1/20 (2006.01); C12N 1/21 (2006.01); C12N 9/78 (2006.01); C12N 9/88 (2006.01); C12N 15/09 (2006.01); C12N 15/55 (2006.01); C12P 13/12 (2006.01)

CPC (source: EP)

C12N 9/78 (2013.01); C12P 13/12 (2013.01)

Citation (search report)

See references of WO 0036120A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

FR 2787121 A1 20000616; FR 2787121 B1 20030912; AU 1568900 A 20000703; AU 773130 B2 20040520; EP 1137784 A1 20011004; JP 2002532096 A 20021002; WO 0036120 A1 20000622

DOCDB simple family (application)

FR 9909489 A 19990719; AU 1568900 A 19991210; EP 99958292 A 19991210; FR 9903089 W 19991210; JP 2000588369 A 19991210