EP 1137784 A1 20011004 - METHOD FOR ISOLATING AND SELECTING GENES CODING FOR ENZYMES, AND SUITABLE CULTURE MEDIUM
Title (en)
METHOD FOR ISOLATING AND SELECTING GENES CODING FOR ENZYMES, AND SUITABLE CULTURE MEDIUM
Title (de)
VERFAHREN ZUR ISOLIERUNG UND SELEKTION VON FÜR ENZYME-KODIERENDE GENE, UND DAFÜR GEEIGNETES KULTURMEDIUM
Title (fr)
METHODE D'ISOLEMENT ET DE SELECTION DE GENES CODANT POUR DES ENZYMES, ET MILIEU DE CULTURE APPROPRIE
Publication
Application
Priority
- FR 9903089 W 19991210
- FR 9815849 A 19981211
- FR 9909489 A 19990719
Abstract (en)
[origin: FR2787121A1] The invention concerns a method for selecting and/or isolating DNA sequences coding for enzymes involved in the biological conversion of an appropriate substrate consisting of methionine and its derivatives, such as HMTBS, said method comprising the following steps: 1) cloning DNA sequences in a carrier enabling their expression in an appropriate host micro-organism; 2) transforming a suitable auxotroph micro-organisms for methionine by introducing carriers previously obtained in said suitable micro-organism; 3) growing the transformed micro-organisms previously obtained in a suitable culture medium comprising a sufficient amount of appropriate substrate; and 4) selecting the transformed micro-organisms capable of growing in the appropriate culture medium; and 5) isolating and as the case may be identifying the DNA sequences involved in the biological conversion of the appropriate substrate.
IPC 1-7
IPC 8 full level
C12N 1/20 (2006.01); C12N 1/21 (2006.01); C12N 9/78 (2006.01); C12N 9/88 (2006.01); C12N 15/09 (2006.01); C12N 15/55 (2006.01); C12P 13/12 (2006.01)
CPC (source: EP)
C12N 9/78 (2013.01); C12P 13/12 (2013.01)
Citation (search report)
See references of WO 0036120A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
FR 2787121 A1 20000616; FR 2787121 B1 20030912; AU 1568900 A 20000703; AU 773130 B2 20040520; EP 1137784 A1 20011004; JP 2002532096 A 20021002; WO 0036120 A1 20000622
DOCDB simple family (application)
FR 9909489 A 19990719; AU 1568900 A 19991210; EP 99958292 A 19991210; FR 9903089 W 19991210; JP 2000588369 A 19991210