EP 1141381 A1 20011010 - METHOD FOR SCREENING FOR INHIBITORS OF RIBOFLAVIN BIOSYNTHESIS
Title (en)
METHOD FOR SCREENING FOR INHIBITORS OF RIBOFLAVIN BIOSYNTHESIS
Title (de)
METHODE ZUM SCREENING VON INHIBITOREN DER RIBOFLAVIN-BIOSYNTHESE
Title (fr)
PROCEDE DE DETECTION D'INHIBITEURS DE BIOSYNTHESE DE LA RIBOFLAVINE
Publication
Application
Priority
- DE 19857868 A 19981215
- DE 19942174 A 19990903
- EP 9909936 W 19991214
Abstract (en)
[origin: WO0040744A1] Methods are described for screening for the presence or absence of inhibition of the activity of enzymes participating in riboflavin biosynthesis. GTP cyclohydrolase II activity is determined through the extent of conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate, catalysed by a protein having a GTP cyclohydrolase II sequence. The activity of 3,4-dihydroxy-2-butanone 4-phosphate synthase activity is detected through the extent of conversion of ribulose 5-phosphate to 3,4-dihydroxy-2-butanone 4-phosphate, catalysed by a protein having a 3,4-dihydroxy-2-butanone 4-phosphate synthase sequence.
IPC 1-7
IPC 8 full level
C12N 9/00 (2006.01); C12N 9/14 (2006.01); C12N 9/78 (2006.01); C12N 9/88 (2006.01); C12N 9/99 (2006.01); C12N 15/09 (2006.01); C12Q 1/25 (2006.01); C12Q 1/34 (2006.01); G01N 33/15 (2006.01); G01N 33/50 (2006.01)
CPC (source: EP)
C12N 9/00 (2013.01); C12N 9/78 (2013.01); C12N 9/88 (2013.01); C12Q 1/25 (2013.01); C12Q 1/34 (2013.01); C07K 2319/00 (2013.01); G01N 2333/978 (2013.01); G01N 2500/20 (2013.01)
Citation (search report)
See references of WO 0040744A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0040744 A1 20000713; AU 1979500 A 20000724; EP 1141381 A1 20011010; JP 2002534094 A 20021015
DOCDB simple family (application)
EP 9909936 W 19991214; AU 1979500 A 19991214; EP 99963535 A 19991214; JP 2000592437 A 19991214