EP 1141397 A1 20011010 - METHOD OF SEQUENCE IDENTIFICATION
Title (en)
METHOD OF SEQUENCE IDENTIFICATION
Title (de)
VERFAHREN FÜR SEQUENZ IDENTIFIZIERUNG
Title (fr)
PROCEDE D'IDENTIFICATION DE SEQUENCE
Publication
Application
Priority
- EP 99962397 A 19991222
- EP 98310567 A 19981222
- GB 9904352 W 19991222
Abstract (en)
[origin: WO0037676A1] Cells of Bacillus subtilis or other micro-organism suitable for screening compounds for antibiotic activity express an essential protein and have a polynucleotide construct comprising a regulatory sequence operably linked to a reporter gene, in which the regulatory sequence is associated with a feedback mechanism responsive to alteration of synthesis or activity of the essential protein. A method of screening compounds for antibiotic or other biological activity comprises incubating the compounds with aliquots of the cells as defined and observing the level of expression of the reporter gene. Methods of identifying suitable regulatory sequences which are not promoters of the target gene, are also described.
IPC 1-7
IPC 8 full level
G01N 33/53 (2006.01); A61K 45/00 (2006.01); A61P 31/04 (2006.01); C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12Q 1/04 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6897 (2018.01); G01N 33/566 (2006.01); C12R 1/125 (2006.01)
CPC (source: EP)
A61P 31/04 (2017.12); C12N 15/1086 (2013.01); C12Q 1/6897 (2013.01)
Citation (search report)
See references of WO 0037676A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0037676 A1 20000629; EP 1141397 A1 20011010; JP 2002533095 A 20021008
DOCDB simple family (application)
GB 9904352 W 19991222; EP 99962397 A 19991222; JP 2000589729 A 19991222