EP 1141401 A1 20011010 - METHOD FOR SEQUENCING DNA USING A MICROFLUIDIC DEVICE
Title (en)
METHOD FOR SEQUENCING DNA USING A MICROFLUIDIC DEVICE
Title (de)
VERFAHREN ZUR SEQUENZIERUNG VON DNA MITTELS EINER MIKROFLUIDICVORRICHTUNG
Title (fr)
PROCEDE DE SEQUEN AGE D'ADN A L'AIDE D'UN DISPOSITIF MICROFLUIDIQUE
Publication
Application
Priority
- EP 9910347 W 19991223
- GB 9828785 A 19981230
Abstract (en)
[origin: WO0040750A1] The present invention describes a method for sequencing DNA in a device with microfluidics properties and a set of reagents for its use. This microfluidic device may be in form of a disc with radially extending microchannel structures (CD form) having an inner application area that may be common for one or more microchannel structures. By spinning the disc the liquid can be driven from an applicator area into reaction and/or detection are as closer to the periphery of the disc. Liquid transportation may thus be driven by centripetal force. The microfluidic device may also have other gemoetrical forms. Several methods can be used to determine the sequence of DNA according to the invention but the real time determination of released pyrophosphate using the luciferase luciferin reaction is preferred.
IPC 1-7
IPC 8 full level
B29C 59/16 (2006.01); C12M 1/00 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6869 (2018.01); G01N 33/53 (2006.01); G01N 33/566 (2006.01)
CPC (source: EP)
B29C 59/16 (2013.01); C12Q 1/6869 (2013.01)
Citation (search report)
See references of WO 0040750A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0040750 A1 20000713; AU 1983800 A 20000724; CA 2357775 A1 20000713; EP 1141401 A1 20011010; GB 9828785 D0 19990217; JP 2002534096 A 20021015
DOCDB simple family (application)
EP 9910347 W 19991223; AU 1983800 A 19991223; CA 2357775 A 19991223; EP 99963601 A 19991223; GB 9828785 A 19981230; JP 2000592442 A 19991223