EP 1141417 A4 20030409 - A METHOD OF AMPLIFICATION OF A CIRCULARISED NUCLEIC ACID PROBE
Title (en)
A METHOD OF AMPLIFICATION OF A CIRCULARISED NUCLEIC ACID PROBE
Title (de)
METHODE ZUR AMPLIFIKATION EINER ZIRKULÄREN NUKLEINSÄURESONDE
Title (fr)
PROCEDE D'AMPLIFICATION D'UNE SONDE D'ACIDE NUCLEIQUE CIRCULARISEE
Publication
Application
Priority
- AU 9901110 W 19991214
- US 11237098 P 19981215
Abstract (en)
[origin: WO0036141A1] A method for amplifying a circular nucleic acid probe produced following interaction of a nucleic acid probe with a target sequence whereby the circular nucleic acid probe is enriched prior to amplification. Enrichment reduces the level of background amplification by removing any linear nucleic acid probes, and may be enzymatic or non-enzymatic. Amplification may be by rolling circle amplification. The probe may be a padlock probe. The terminal sequences of the probe may form non-contiguous duplexes with the probe circularised through ligation of a capture ligand or spacer nucleic acid molecule between the two terminal sequences. The capture ligand or spacer nucleic acid molecule may be labelled, such as with biotin. The method is used in applications involving the detection of specific nucleic acid sequences.
IPC 1-7
IPC 8 full level
G01N 33/50 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12Q 1/682 (2018.01); G01N 33/53 (2006.01); G01N 33/566 (2006.01)
CPC (source: EP)
C12Q 1/682 (2013.01)
C-Set (source: EP)
Citation (search report)
- [T] VOISEY J ET AL: "Interrogation of multimeric DNA amplification products by competitive primer extension using bst DNA polymerase (large fragment).", BIOTECHNIQUES. UNITED STATES NOV 2001, vol. 31, no. 5, November 2001 (2001-11-01), pages 1122 - 1124, 1126, 1128 - 1129, XP002228714, ISSN: 0736-6205
- [AD] LIZARDI P M ET AL: "MUTATION DETECTION AND SINGLE-MOLECULE COUNTING USING ISOTHERMAL ROLLING-CIRCLE AMPLIFICATION", NATURE GENETICS, NEW YORK, NY, US, vol. 19, no. 3, July 1998 (1998-07-01), pages 225 - 232, XP000857368, ISSN: 1061-4036
- See references of WO 0036141A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0036141 A1 20000622; AU 2781900 A 20000703; AU 781962 B2 20050623; CA 2394800 A1 20000622; EP 1141417 A1 20011010; EP 1141417 A4 20030409; JP 2002532102 A 20021002; JP 4163386 B2 20081008
DOCDB simple family (application)
AU 9901110 W 19991214; AU 2781900 A 19991214; CA 2394800 A 19991214; EP 99969209 A 19991214; JP 2000588388 A 19991214