EP 1144092 A4 20021029 - INTEGRATED PORTABLE BIOLOGICAL DETECTION SYSTEM
Title (en)
INTEGRATED PORTABLE BIOLOGICAL DETECTION SYSTEM
Title (de)
INTEGRIERTES TRAGBARES SYSTEM ZUR BIOLOGISCHEN DETEKTION
Title (fr)
SYSTEME DE DETECTION BIOLOGIQUE INTEGRE PORTATIF
Publication
Application
Priority
- US 9931098 W 19991222
- US 11373098 P 19981223
Abstract (en)
[origin: WO0037163A1] We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by stining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the contruction of a "laboratory-on-a-chip" system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.
IPC 1-7
IPC 8 full level
G01N 21/64 (2006.01); B01D 57/02 (2006.01); B03C 5/00 (2006.01); C12M 1/00 (2006.01); C12M 1/33 (2006.01); C12M 3/08 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C40B 40/02 (2006.01); C40B 50/06 (2006.01); G01N 21/78 (2006.01); G01N 27/447 (2006.01); G01N 33/48 (2006.01); G01N 33/53 (2006.01); G01N 33/566 (2006.01); G01N 37/00 (2006.01); C12R 1/19 (2006.01)
CPC (source: EP KR)
G01N 15/1433 (2024.01 - EP KR); G01N 15/1484 (2013.01 - KR); G01N 33/5438 (2013.01 - EP KR); G01N 15/1484 (2013.01 - EP)
Citation (search report)
- [DPX] WO 9938612 A1 19990805 - NANOGEN INC [US]
- [Y] WO 9702357 A1 19970123 - AFFYMETRIX INC [US], et al & US 5856174 A 19990105 - LIPSHUTZ ROBERT J [US], et al
- [A] WO 9804355 A1 19980205 - BRITISH TECH GROUP [GB], et al
- [DXY] CHENG, J., ET AL.: "Isolation of cultured cervical carcinoma cells mixed with peripheral blood cells on a bioelectronic chip", ANALYTICAL CHEMISTRY, vol. 70, no. 11, 1 June 1998 (1998-06-01), pages 2321 - 2326, XP002216911
- [DX] CHENG, J., ET AL.: "Preparation and hybridization analysis of DNA/RNA from E.coli on microfabricated biolelctronic chips", NATURE BIOTECHNOLOGY, vol. 16, 1 June 1998 (1998-06-01), pages 541 - 546, XP001118221
- See also references of WO 0037163A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0037163 A1 20000629; AU 2595000 A 20000712; AU 763514 B2 20030724; BR 9916840 A 20011009; CA 2353461 A1 20000629; EP 1144092 A1 20011017; EP 1144092 A4 20021029; JP 2002536962 A 20021105; KR 20010102961 A 20011117; NZ 512087 A 20030530
DOCDB simple family (application)
US 9931098 W 19991222; AU 2595000 A 19991222; BR 9916840 A 19991222; CA 2353461 A 19991222; EP 99968558 A 19991222; JP 2000589268 A 19991222; KR 20017007947 A 20010622; NZ 51208799 A 19991222