EP 1153300 A2 20011114 - LCM (LASER CAPTURE MICRODISSECTION) FOR CELLULAR PROTEIN ANALYSIS
Title (en)
LCM (LASER CAPTURE MICRODISSECTION) FOR CELLULAR PROTEIN ANALYSIS
Title (de)
ANALYSE VON ZELLULÄREN PROTEINGEHALT UNTER VERWENDUNG VON LCM (LASER CAPTURE MICRODISSECTION)
Title (fr)
PROCEDES ET DISPOSITIFS D'ISOLATION ET D'ANALYSE DE LA TENEUR PROTEIQUE DES CELLULES
Publication
Application
Priority
- US 0004023 W 20000216
- US 12028899 P 19990216
Abstract (en)
[origin: WO0049410A2] The present invention describes devices and methods for performing protein analysis on laser capture microdissected cells, which permits proteomic analysis on cells of different populations. Particular disclosed examples are analysis of normal versus malignant cells, or a comparison of differential protein expression in cells that are progressing from normal to malignant. The protein content of the microdissected cells may be analyzed using techniques such as immunoassays, 1D and 2D gel electrophoresis characterization, Western blotting, liquid chromatography quadrapole ion trap electrospray (LCQ-MS), Matrix Assisted Laser Desorption Ionization/Time of Flight (MALDI/TOF), and Surface Enhanced Laser Desorption Ionization Spectroscopy (SELDI). In addition to permitting direct comparison of qualitative and quantitative protein content of tumor cells and normal cells from the same tissue sample, the methods also allow for investigation of protein characteristics of tumor cells, such as binding ability and amino acid sequence, and differential expression of proteins in particular cell populations in response to drug treatment. The present methods also provide, through the use of protein fingerprinting, a rapid and reliable way to identify the source tissue of a tumor metastasis.
IPC 1-7
IPC 8 full level
G01N 1/28 (2006.01); G01N 1/30 (2006.01); G01N 33/68 (2006.01)
CPC (source: EP)
G01N 1/2813 (2013.01); G01N 1/30 (2013.01); G01N 33/6803 (2013.01); G01N 2001/284 (2013.01); G01N 2550/00 (2013.01); H01J 49/00 (2013.01)
Citation (search report)
See references of WO 0049410A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0049410 A2 20000824; WO 0049410 A3 20010308; WO 0049410 A9 20010830; AU 3367100 A 20000904; AU 772680 B2 20040506; CA 2359649 A1 20000824; EP 1153300 A2 20011114; JP 2002537561 A 20021105
DOCDB simple family (application)
US 0004023 W 20000216; AU 3367100 A 20000216; CA 2359649 A 20000216; EP 00911845 A 20000216; JP 2000600101 A 20000216