EP 1155026 A2 20011121 - METHOD FOR ISOLATING NUCLEIC ACIDS
Title (en)
METHOD FOR ISOLATING NUCLEIC ACIDS
Title (de)
VERFAHREN ZUR ISOLIERUNG VON NUCLEINSÄUREN
Title (fr)
PROCEDE D'ISOLEMENT D'ACIDES NUCLEIQUES
Publication
Application
Priority
- DE 19907023 A 19990219
- EP 0001028 W 20000209
Abstract (en)
[origin: DE19907023A1] A method for isolation of nucleic acids, comprising adsorption onto polymeric beads at neutral or acidic pH then release at basic pH, is new. Method for isolating nucleic acid (NA) comprises: (i) mixing a sample at pH 7 or below with a polymer (A) that is insoluble in water and nonionic at basic and neutral pH to adsorb NA; (ii) separating (A) and (iii) treating (A) with an aqueous phase at pH over 7 to release NA. (A) consists of beads of particle size 3-100 micron prepared from 5-98% amino-monomer (AM), 0.3-90% crosslinker (CL) and 0-93% vinyl monomer (VM), by weight. Independent claims are also included for the following: (1) macroporous polymer beads (A') of particle size 3-100 micron, pore size 10-1000 nm and specific surface area (measured by the BET method) 5-500 square m/g and prepared from 5-98% AM, 2-30% CL and 0-93% hydrophobic VM; (2) water-insoluble but water-swellable polymer beads (A'') of particle size 3-100 micron and prepared from 5-79.7% AM, 0.3-10% CL and 10-93% hydrophilic VM; (3) a method for preparing (A') and (A''); (4) the amino-monomer of formula (I); (5) a method for preparing (I); and (6) an agent for isolation of NA comprising (A') or (A'').
IPC 1-7
C07H 21/00; C08J 9/20; C08F 20/36; C08F 220/36; C07D 233/48; C12Q 1/68
IPC 8 full level
C07D 233/61 (2006.01); C07H 21/04 (2006.01); C12N 15/10 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6806 (2018.01)
CPC (source: EP)
C12N 15/1006 (2013.01); C12Q 1/6806 (2013.01)
Citation (search report)
See references of WO 0049031A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
DE 19907023 A1 20000824; AU 2547100 A 20000904; CA 2362979 A1 20000824; EP 1155026 A2 20011121; JP 2002537306 A 20021105; WO 0049031 A2 20000824; WO 0049031 A3 20010412
DOCDB simple family (application)
DE 19907023 A 19990219; AU 2547100 A 20000209; CA 2362979 A 20000209; EP 0001028 W 20000209; EP 00903676 A 20000209; JP 2000599768 A 20000209